Barrow B J, Ortiz-Reyes R, O'Riordan M A, Pretlow T P
Institute of Pathology, Case Western Reserve University, Cleveland, Ohio 44106.
Histochem J. 1989 May;21(5):289-95. doi: 10.1007/BF01757182.
Several enzymes were investigated histochemically in the colons of normal male F344 rats in order to understand the function of different types of cells in this tissue. Serial methacrylate-embedded sections (2-4 microns) allowed the precise localizations of several enzymes including acid phosphatase, alkaline phosphatase, gamma-glutamyl transpeptidase, N-acetyl-beta-D-glucosaminidase (hexosaminidase), alpha-naphthyl butyrate esterase and 5'-nucleotidase. Sites reactive with periodic acid-Schiff were also localized. Gradients of enzyme activity were observed between caecum and rectum and/or from the luminal surfaces to the bases of the crypts for hexosaminidase, esterase and gamma-glutamyl transpeptidase. To our knowledge this is the first histochemical demonstration of gamma-glutamyl transpeptidase in normal rat colonic epithelial cells. The utilization of the methacrylate-embedding technique has revealed previously undescribed gradients of enzyme activity and has allowed the localization of enzyme activities not previously reported in normal rat colonic mucosa.
为了解正常雄性F344大鼠结肠中不同类型细胞的功能，对几种酶进行了组织化学研究。连续的甲基丙烯酸酯包埋切片（2 - 4微米）能够精确地定位几种酶，包括酸性磷酸酶、碱性磷酸酶、γ-谷氨酰转肽酶、N-乙酰-β-D-氨基葡萄糖苷酶（己糖胺酶）、α-萘丁酸酯酶和5'-核苷酸酶。还定位了与过碘酸-希夫反应的位点。对于己糖胺酶、酯酶和γ-谷氨酰转肽酶，在盲肠和直肠之间以及/或者从隐窝的腔面到基部观察到了酶活性梯度。据我们所知，这是γ-谷氨酰转肽酶在正常大鼠结肠上皮细胞中的首次组织化学证明。甲基丙烯酸酯包埋技术的应用揭示了以前未描述的酶活性梯度，并使得在正常大鼠结肠黏膜中以前未报道的酶活性得以定位。
