Freeman H J, Lotan R, Kim Y S
Lab Invest. 1980 Apr;42(4):405-12.
Paraffin-embedded mucosal biopsies from proximal and distal colon of male Wistar rats were examined by fluorescence microscopy after labeling with nine fluorescein-conjugated lectins. These were isolated by affinity column chromatography, conjugated to fluorescein isothiocyanate, and used as specific molecular probes to define the labeling patterns of carbohydrate-containing components of colonic goblet cells at both sites. Differential lectin labeling of goblet cells within the colonic crypts was observed in both regions likely reflecting increasing differentiation of these epithelial cells. Furthermore, marked regional differences in goblet cell mucus labeling were also detected, indicating that the carbohydrate components of these mucosal cells differ significantly between proximal and distal colon of the rat. These regional differences suggest that there are significant differences in the biologic characteristics of colonic mucosa from these two sites.
用九种异硫氰酸荧光素偶联凝集素标记后，通过荧光显微镜检查雄性Wistar大鼠近端和远端结肠石蜡包埋的黏膜活检组织。这些凝集素通过亲和柱层析分离，与异硫氰酸荧光素偶联，并用作特异性分子探针，以确定两个部位结肠杯状细胞含碳水化合物成分的标记模式。在两个区域均观察到结肠隐窝内杯状细胞的凝集素标记差异，这可能反映了这些上皮细胞分化程度增加。此外，还检测到杯状细胞黏液标记存在明显的区域差异，表明大鼠近端和远端结肠这些黏膜细胞的碳水化合物成分存在显著差异。这些区域差异表明，这两个部位的结肠黏膜生物学特性存在显著差异。
