Nasri Rim, Jridi Mourad, Lassoued Imen, Jemil Ines, Ben Slama-Ben Salem Rabeb, Nasri Moncef, Karra-Châabouni Maha
Laboratory of Microbiology and Enzyme Engineering, Sfax University, National School of Engineering, B.P 1173-3038, Sfax, Tunisia,
Appl Biochem Biotechnol. 2014 Jul;173(5):1121-34. doi: 10.1007/s12010-014-0905-3. Epub 2014 Apr 25.
Antioxidant properties and angiotensin-converting enzyme (ACE) inhibitory activities of protein hydrolysates from goby (Zosterisessor ophiocephalus) muscle, with different degrees of hydrolysis (DH) from 5 to 25%, prepared by treatment with crude proteases extract from smooth hound intestines, were investigated. Goby protein hydrolysates (GPHs) are rich in Gly and Thr, which accounted for 14.1-15% and 11.6-13.2% of the total amino acids, respectively. The antioxidant activities of GPHs were investigated by using several in vitro assay systems. All GPHs exhibited significant metal chelating activity and DPPH free radical-scavenging activity, and inhibited linoleic acid peroxidation. For the ACE-inhibitory activity, as the DH increased, the activity of GPHs increased. The obtained results revealed that antioxidant and ACE-inhibitory activities of GPHs were influenced by the degree of hydrolysis. A medium degree of enzymatic hydrolysis was appropriate to obtain GPHs with good antioxidant activity, while small peptides were essential to obtain high ACE inhibitory activity.
研究了用平滑狗鲨肠道粗蛋白酶提取物制备的不同水解度（5%至25%）的虾虎鱼（纹缟虾虎鱼）肌肉蛋白水解物的抗氧化特性和血管紧张素转换酶（ACE）抑制活性。虾虎鱼蛋白水解物（GPHs）富含甘氨酸和苏氨酸，分别占总氨基酸的14.1 - 15%和11.6 - 13.2%。通过几种体外分析系统研究了GPHs的抗氧化活性。所有GPHs均表现出显著的金属螯合活性和DPPH自由基清除活性，并抑制亚油酸过氧化。对于ACE抑制活性，随着水解度的增加，GPHs的活性增加。所得结果表明，GPHs的抗氧化和ACE抑制活性受水解度的影响。中等程度的酶水解适合获得具有良好抗氧化活性的GPHs，而小肽对于获得高ACE抑制活性至关重要。
