类固醇5α-还原酶的表达克隆与调控，该酶是雄性性别分化所必需的一种酶。
Expression cloning and regulation of steroid 5 alpha-reductase, an enzyme essential for male sexual differentiation.
作者信息
Andersson S, Bishop R W, Russell D W
机构信息
Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas 75235.
出版信息
J Biol Chem. 1989 Sep 25;264(27):16249-55.
Abstract
The conversion of testosterone into the more potent androgen, dihydrotestosterone, catalyzed by the enzyme steroid 5 alpha-reductase, is required for the differentiation of male external genitalia. Here, we report the isolation of cDNA clones encoding the rat steroid 5 alpha-reductase using expression cloning in Xenopus oocytes. DNA sequence analysis demonstrates that the liver and ventral prostate forms of steroid 5 alpha-reductases are identical hydrophobic proteins of 29 kDa. The amount of steroid 5 alpha-reductase mRNA in liver increased in response to castration, but remained unchanged in the prostate. Testosterone administration to castrates induced expression of mRNA in the prostate but had no effect on liver. The data suggest that the steroid 5 alpha-reductase gene is differentially regulated by testosterone in androgen-responsive versus non-responsive tissues.
摘要
由类固醇5α-还原酶催化的睾酮向活性更强的雄激素双氢睾酮的转化，是男性外生殖器分化所必需的。在此，我们报告了利用非洲爪蟾卵母细胞中的表达克隆技术分离出编码大鼠类固醇5α-还原酶的cDNA克隆。DNA序列分析表明，肝脏和腹侧前列腺形式的类固醇5α-还原酶是相同的29 kDa疏水蛋白。阉割后肝脏中类固醇5α-还原酶mRNA的量增加，但在前列腺中保持不变。给阉割后的大鼠注射睾酮可诱导前列腺中mRNA的表达，但对肝脏没有影响。这些数据表明，类固醇5α-还原酶基因在雄激素反应性组织和非反应性组织中受睾酮的差异调节。
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