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A novel in situ colony screening method to detect human immunodeficiency virus reverse transcriptase activity expressed in bacteria. Isolation of pseudorevertants of reverse transcriptase mutants.

作者信息

Prasad V R, Goff S P

机构信息

Department of Biochemistry, Columbia University College of Physicians and Surgeons, New York, New York 10032.

出版信息

J Biol Chem. 1989 Oct 5;264(28):16689-93.

PMID:2476446
Abstract

An in situ screening procedure is described that permits the detection of RNA-dependent DNA polymerase activity in bacterial colonies expressing the reverse transcriptase of the human immunodeficiency virus. The procedure has been applied to the isolation of rare pseudorevertants of an inactive parental mutant.

摘要

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