Talbot P J, Lapierre J, Daniel C, Dugré R, Trépanier P
Centre de recherche en virologie, Université du Québec, Ville de Laval, Canada.
J Virol Methods. 1989 Jul;25(1):63-70. doi: 10.1016/0166-0934(89)90100-6.
The growth of the murine coronavirus MHV-A59 on murine DBT cells adapted to dextran-made Cytodex 1 microcarriers was studied in comparison with cells grown on plastic dishes. With a microcarrier concentration of 5 g/l in spinner flasks, a density of 3 x 10(6) cells/ml was reached in 7 days. Under these conditions, cells supported virus growth to the same extent as when they were grown on the plastic substratum. This was shown by a similar development of virus-induced syncytia, the release of an equivalent number of infectious progeny virions per cell, similar recoveries observed after concentration and purification and an identical appearance of the purified virus under the electron microscope. On the other hand, the technical convenience of microcarriers and the ease of scale-up emphasize their potential for the growth of coronaviruses.
研究了鼠冠状病毒MHV - A59在适应葡聚糖制成的Cytodex 1微载体的鼠DBT细胞上的生长情况，并与在塑料培养皿上生长的细胞进行了比较。在转瓶中微载体浓度为5 g/l的情况下，7天内细胞密度达到3×10⁶个细胞/ml。在这些条件下，细胞支持病毒生长的程度与在塑料基质上生长时相同。这通过病毒诱导的合胞体的类似发育、每个细胞释放等量的感染性子代病毒粒子、浓缩和纯化后观察到的类似回收率以及纯化病毒在电子显微镜下的相同外观得以证明。另一方面，微载体的技术便利性和易于放大突出了它们在冠状病毒生长方面的潜力。
