用小麦胚凝集素和核糖核酸酶对包埋在环氧树脂中的经锇酸处理的组织进行金标记。
Gold labeling with wheat germ agglutinin and RNAse on osmicated tissue embedded in epoxy resin.
作者信息
Brooks E M, Binnington K C
机构信息
CSIRO, Division of Entomology, Canberra, Australia.
出版信息
J Histochem Cytochem. 1989 Oct;37(10):1557-61. doi: 10.1177/37.10.2476480.
Tissue of an insect, Lucilia cuprina, fixed conventionally in buffered glutaraldehyde and osmium and embedded in epoxy resin (epon or epon/araldite), provided sections which could readily be labeled with RNAse/gold and wheat germ agglutinin (WGA)/gold. This method offers labeling of tissues with improved contrast and allows the retrospective application of RNAse and WGA labeling to conventionally prepared tissues, without recourse to oxidizing/etching agents.
一种昆虫（铜绿蝇）的组织，常规固定于缓冲戊二醛和锇中，包埋于环氧树脂（环氧树脂或环氧树脂/阿拉底特）中，所提供的切片能够很容易地用核糖核酸酶/金和麦胚凝集素（WGA）/金进行标记。该方法能以更高的对比度对组织进行标记，并允许对常规制备的组织进行核糖核酸酶和WGA标记的追溯应用，而无需使用氧化/蚀刻剂。
Zotero 插件