Danscher G, Nörgaard J O
J Histochem Cytochem. 1983 Dec;31(12):1394-8. doi: 10.1177/31.12.6631001.
RNase labeled with colloidal gold was used as a model for the present technique evolved for the light microscopic localization of gold-labeled substances in semithin resin-embedded sections. Tissue sections placed on glass slides were treated with the gold-enzyme complex and subsequently exposed to a photographic developed containing silver lactate. During the development gold particles are encapsulated in growing shells of metallic silver and gradually made visible in the light microscope. The amplification method can be applied to paraffin-embedded and frozen sections as well. This technique may prove useful as a supplement to studies utilizing colloidal gold or silver as markers normally used at the electron microscopic level.
用胶体金标记的核糖核酸酶作为本技术的模型，该技术是为在半薄树脂包埋切片中对金标记物质进行光镜定位而开发的。置于载玻片上的组织切片用金-酶复合物处理，随后暴露于含有乳酸银的显影液中。在显影过程中，金颗粒被包裹在不断生长的金属银壳中，并逐渐在光学显微镜下可见。该放大方法也可应用于石蜡包埋和冰冻切片。作为对利用通常在电子显微镜水平使用的胶体金或银作为标记的研究的补充，这项技术可能证明是有用的。
