Shimono M, Inoue T, Hamada Y, Abiko Y, Hashimoto S
J Periodontal Res. 1989 May;24(3):186-91. doi: 10.1111/j.1600-0765.1989.tb02004.x.
To clarify the details of intracytoplasmic granules in the junctional epithelium (JE) of the rat gingiva, phosphotungstic acid (PTA) staining, acid phosphatase cytochemistry and zinc iodide-osmium (ZIO) staining were carried out. Condensing granules containing homogeneous electron-dense materials, measuring about 260 nm in diameter, have been observed at the peripheral cytoplasm of the JE. These granules reveal PTA-positive, ZIO-positive and acid phosphatase-positive staining. These results indicate that the granules contain glycoprotein, phospholipid, lipoprotein and lysosomal enzymes. From their composition and morphology, it is suggested that these granules in the JE may contribute secretory function and secrete glycoproteins or glycolipids. Although it is unclear whether the dense granules correspond to membrane-coating granules by our staining methods, it is surmised that, if the dense granules can engage in establishment of the barrier function, then this role is impeded by the large intercellular space in the JE.
为阐明大鼠牙龈结合上皮（JE）中胞浆内颗粒的细节，进行了磷钨酸（PTA）染色、酸性磷酸酶细胞化学和碘化锌-锇（ZIO）染色。在JE的周边细胞质中观察到含有均匀电子致密物质的凝聚颗粒，直径约260nm。这些颗粒显示PTA阳性、ZIO阳性和酸性磷酸酶阳性染色。这些结果表明颗粒含有糖蛋白、磷脂、脂蛋白和溶酶体酶。从其组成和形态来看，提示JE中的这些颗粒可能具有分泌功能并分泌糖蛋白或糖脂。尽管通过我们的染色方法尚不清楚致密颗粒是否对应于膜包被颗粒，但据推测，如果致密颗粒能够参与屏障功能的建立，那么JE中较大的细胞间隙会阻碍这一作用。
