Ashrafi S H, Squier C A, Meyer J
Histochem J. 1981 Jan;13(1):45-55. doi: 10.1007/BF01005838.
Some of the parameters affecting the staining of keratinized oral epithelium with the zinc iodide-osmium reaction were examined using light and electron microscopy and electron probe microanalysis. Factors examined were block size, incubation temperature and the effect of aldehyde prefixation. Large blocks (4 mm cube) were subdivided after incubation and the staining of the centre and edge compared. Generally the reaction was more variable at the edge than in the centre. Small block (1 mm cube) showed a more intense reaction when incubated at 24 degrees C than at 4 degrees C. In all these preparations, final reaction product was seen over Golgi systems, lysosome-like bodies, membrane-coating granules and, in the more intensely stained regions, over endoplasmic reticulum and nuclear membranes as well. In prefixed material, mitochondria were frequently stained in addition to the other organelles. Energy dispersive analysis showed the reaction product to be similar in all preparations and to contain high levels of zinc and osmium but not iodine.
运用光学显微镜、电子显微镜以及电子探针微量分析技术，对一些影响碘化锌 - 锇反应对角化口腔上皮染色的参数进行了研究。所考察的因素包括组织块大小、孵育温度以及醛类预固定的影响。孵育后，将大组织块（4立方毫米）进行细分，并比较中心部位和边缘部位的染色情况。一般来说，边缘部位的反应比中心部位更具变异性。小组织块（1立方毫米）在24摄氏度孵育时比在4摄氏度孵育时显示出更强的反应。在所有这些标本中，在高尔基体系统、溶酶体样小体、膜包被颗粒上以及在染色较深的区域，在内质网和核膜上也可见到最终反应产物。在预固定的材料中，除了其他细胞器外，线粒体也经常被染色。能谱分析表明，所有标本中的反应产物相似，且含有高水平的锌和锇，但不含碘。
