Lu K S, Chiang H N, Lin H S
Department of Anatomy, College of Medicine, National Taiwan University, Taipei, Republic of China.
Proc Natl Sci Counc Repub China B. 1987 Jul;11(3):297-306.
The subcommissural organ (SCO) of the golden hamster (Mesocricetus auratus) was studied by conventional electron microscopy, freeze-fracture technique, zinc-iodide-osmium (ZIO) and acid phosphatase cytochemical reactions. The ultrastructure of hamster SCO cells shows a few flattened cisternae of rough endoplasmic reticulum (ER) without dilated ones in the cytoplasm. The Golgi apparatus is very well developed. Freeze-fracture studies also indicate only short profiles of flattened ER in the cytoplasm endorsing the absence of dilated ER cisternae. After the treatment with ZIO mixture, reaction products were observed over flattened cisternae of the ER and the nuclear envelope. The Golgi apparatus was also reactive toward the ZIO mixture. Acid phosphatase activities are localized in the inner one or two saccules of the Golgi apparatus and dense bodies. From these results we suggest that (1) hamster SCO cells do not accumulate secretory material in the cytoplasm in the form of discrete secretory granules or dilated cisternae of ER, and (2) hamster SCO cells may possess extremely high secretory activity or may not be actively involved in secretory function at all as in rats or other rodents.
采用传统电子显微镜、冷冻蚀刻技术、碘化锌锇（ZIO）和酸性磷酸酶细胞化学反应，对金黄仓鼠（Mesocricetus auratus）的室管膜下器官（SCO）进行了研究。仓鼠SCO细胞的超微结构显示，细胞质中有一些扁平的粗面内质网（ER）池，但没有扩张的池。高尔基体非常发达。冷冻蚀刻研究还表明，细胞质中只有扁平内质网的短轮廓，这证实了不存在扩张的内质网池。用ZIO混合物处理后，在内质网的扁平池和核膜上观察到反应产物。高尔基体对ZIO混合物也有反应。酸性磷酸酶活性定位于高尔基体的内一两个囊泡和致密体中。从这些结果我们推测：（1）仓鼠SCO细胞不以离散分泌颗粒或内质网扩张池的形式在细胞质中积累分泌物质；（2）仓鼠SCO细胞可能具有极高的分泌活性，或者可能根本不像大鼠或其他啮齿动物那样积极参与分泌功能。
