Sellrie Frank, Lenz Christine, Andersson Anika, Wilhelmsson L Marcus, Schenk Jörg A
UP Transfer GmbH, Am Neuen Palais 10, Potsdam, Germany.
UP Transfer GmbH, Am Neuen Palais 10, Potsdam, Germany.
Talanta. 2014 Jun;124:67-70. doi: 10.1016/j.talanta.2014.02.046. Epub 2014 Feb 28.
We report on the generation and analytical application of the monoclonal antibody G93-ED2 raised against the tricyclic fluorescent nucleoside analogue 1,3-diaza-2-oxophenoxazine (tC°). G93-ED2 is specifically binding this deoxycytidine analogue and was found to raise its fluorescence intensity by a factor of 5. This unique feature makes it a valuable tool in fluorescence dependent immunoassays. G93-ED2 was successfully applied in a homogeneous fluorescence quenching immunoassay (DNA-Q) for the sequence specific determination of DNA.
我们报道了针对三环荧光核苷类似物1,3 - 二氮杂 - 2 - 氧代吩恶嗪（tC°）产生的单克隆抗体G93 - ED2及其分析应用。G93 - ED2能特异性结合这种脱氧胞苷类似物，并发现可使其荧光强度提高5倍。这一独特特性使其成为荧光依赖免疫分析中有价值的工具。G93 - ED2已成功应用于一种用于DNA序列特异性测定的均相荧光猝灭免疫分析（DNA - Q）中。
