School of Environmental Science and Engineering, Pohang University of Science and Technology, Pohang, Gyeongbuk 790-784, Republic of Korea.
Infrastructure and Environment Division, School of Engineering, University of Glasgow, Glasgow G12 8LT, UK.
Bioresour Technol. 2014 Sep;168:59-63. doi: 10.1016/j.biortech.2014.03.161. Epub 2014 Apr 8.
Methanogen communities were investigated using 454 pyrosequencing in four different full-scale anaerobic digesters treating food waste-recycling wastewater. Seasonal samples were collected for 2 years, and 24 samples were available for microbial analysis from a plug flow thermophilic (PT) digester, a continuously-stirred tank thermophilic (CT) digester, an upflow anerobic sludge blanket mesophilic (UM) digester, and a continuously-stirred tank mesophilic (CM) digester. Methanoculleus, Methanobacterium, Methanothermobacter, and Methanosaeta were revealed to be key methanogens in full-scale anaerobic digestion process treating food waste-recycling wastewater. In the PT digester, Methanoculleus was dominant (96.8%). In the CT digester, Methanoculleus was dominant (95.4%) during the first year of operation, but the dominant genus was shifted to Methanothermobacter (98.5%) due to pH increase. In the UM digester, Methanosaeta was dominant (87.2%). In the CM digester, Methanoculleus was constantly dominant (74.8%) except during CM5 when Methanosaeta was dominant (62.6%) due to the low residual acetate concentration (0.1 g/L).
采用 454 焦磷酸测序技术对处理食物垃圾再循环废水的四个不同规模的厌氧消化器中的产甲烷菌群落进行了研究。在两年内采集了季节性样本，从推流式高温（PT）消化器、连续搅拌式高温（CT）消化器、上流式厌氧污泥床中温（UM）消化器和连续搅拌式中温（CM）消化器中获得了 24 个微生物分析样本。产甲烷菌 Methanoculleus、Methanobacterium、Methanothermobacter 和 Methanosaeta 被揭示是处理食物垃圾再循环废水的大规模厌氧消化过程中的关键产甲烷菌。在 PT 消化器中，Methanoculleus 占主导地位（96.8%）。在 CT 消化器中，Methanoculleus 在运行的第一年占主导地位（95.4%），但由于 pH 值升高，优势属被转移到 Methanothermobacter（98.5%）。在 UM 消化器中，Methanosaeta 占主导地位（87.2%）。在 CM 消化器中，Methanoculleus 一直占主导地位（74.8%），除了 CM5 之外，由于残留乙酸浓度较低（0.1 g/L），Methanosaeta 占主导地位（62.6%）。
