School of Environmental Science and Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk, South Korea.
Department of Environmental Engineering, Kyungpook National University, Daegu, South Korea.
Bioresour Technol. 2017 Mar;228:368-373. doi: 10.1016/j.biortech.2016.12.118. Epub 2017 Jan 4.
Four full-scale mesophilic anaerobic digesters treating waste sludge were monitored to characterize methanogen communities and their relationship with process parameters. The performance of the four digesters were dissimilar with the average chemical oxygen demand removal efficiencies between 24 and 45% and differing pH. Real-time quantitative PCR showed that archaeal 16S rRNA gene concentration ([ARC]) and, more pronouncedly, its ratio to bacterial counterpart ([ARC]/[BAC]) correlated positively with the performance parameters, including the lipid removal efficiency. Pyrosequencing identified 12 methanogen genera, of which Methanolinea, Methansaeta, and Methanospirillum collectively accounted for 79.2% of total archaeal reads. However, Methanoculleus, a numerically minor (1.9±2.6%) taxa, was the most promising biomarker for positive performance, while Methanoregula was abundant in samples with poor performance. These results could be useful for the control and management of anaerobic sludge digestion.
四个处理剩余污泥的中温厌氧消化池被监测以描述产甲烷菌群落及其与工艺参数的关系。四个消化池的性能不同，平均化学需氧量去除效率在 24%至 45%之间，pH 值也不同。实时定量 PCR 显示古菌 16S rRNA 基因浓度 ([ARC])，更明显的是，其与细菌对应物的比值 ([ARC]/[BAC]) 与性能参数呈正相关，包括油脂去除效率。焦磷酸测序鉴定出 12 种产甲烷菌属，其中 Methanolinea、Methansaeta 和 Methanospirillum 共同占总古菌读数的 79.2%。然而，数量较少（1.9±2.6%）的 Methanoculleus 是性能良好的最有希望的生物标志物，而 Methanoregula 在性能不佳的样本中丰度较高。这些结果可能对厌氧污泥消化的控制和管理有用。
