将限制性内切酶MspI、HpaII、HaeIII和HinfI应用于鸡有丝分裂染色体。

Restriction enzymes MspI, HpaII, HaeIII, and HinfI applied to chicken mitotic chromosomes.

作者信息

Opitz G, Guise K S, Otis J S, Shoffner R N

机构信息

Department of Animal Science, University of Minnesota, St. Paul 55108.

出版信息

Poult Sci. 1989 Jul;68(7):1027-9. doi: 10.3382/ps.0681027.

DOI:10.3382/ps.0681027

PMID:2476797

Abstract

The restriction enzymes MspI, HpaII, HaeIII, and HinfI were applied in situ to chicken metaphase chromosomes to determine if they denatured specific regions to produce banding patterns. Human metaphase chromosomes were treated simultaneously to serve as controls. The MspI, HpaII, and HaeIII enzymes produced no visible banding patterns in chicken chromosomes. There was slight banding on a few chromosomes in HinfI preparations, which was similar to the occasional spontaneous banding observed in chicken chromosome preparations. The results indicate that these four enzymes do not denature C-band regions in sufficient magnitude to be detected in chicken metaphase chromosomes.

摘要

将限制性内切酶MspI、HpaII、HaeIII和HinfI原位应用于鸡中期染色体，以确定它们是否使特定区域变性从而产生带型。同时处理人类中期染色体作为对照。MspI、HpaII和HaeIII酶在鸡染色体中未产生可见的带型。在HinfI处理的样本中，少数染色体上有轻微的带型，这与在鸡染色体样本中偶尔观察到的自发带型相似。结果表明，这四种酶不会使鸡中期染色体中的C带区域变性到足以被检测到的程度。