Adolph S, Hameister H
Abteilung Klinische Genetick der Universität, Ulm, FRG.
Cytogenet Cell Genet. 1990;54(3-4):132-6. doi: 10.1159/000132976.
Several restriction enzymes (HindIII, HaeIII, MspI, HpaII, EcoRI, KpnI, and NotI) were evaluated for their ability to induce bands in human metaphase chromosomes during in situ nick translation. MspI and HpaII were able to induce a completely developed R-band pattern. Preferential cleavage of R-band chromatin is due to the presence of unmethylated CpG-residues present in CpG-rich islands, which are apparently unevenly distributed and mainly concentrated in R-bands.
对几种限制性内切酶（HindIII、HaeIII、MspI、HpaII、EcoRI、KpnI和NotI）进行了评估，以考察它们在原位缺口平移过程中诱导人类中期染色体出现条带的能力。MspI和HpaII能够诱导出完全显现的R带模式。R带染色质的优先切割是由于富含CpG的岛中存在未甲基化的CpG残基，这些残基分布明显不均，主要集中在R带中。
