Zhao Yi, Chen Xuemei, Liu Xueqing, Ding Yubin, Gao Rufei, Qiu Yiwen, Wang Yingxiong, He Junlin
Laboratory of Reproductive Biology, School of Public Health, Chongqing Medical University, Box 197, No. 1 Yixueyuan Road, Yuzhong District, 400016 Chongqing, PR China.
Laboratory of Reproductive Biology, School of Public Health, Chongqing Medical University, Box 197, No. 1 Yixueyuan Road, Yuzhong District, 400016 Chongqing, PR China.
Food Chem Toxicol. 2014 Jul;69:244-51. doi: 10.1016/j.fct.2014.04.021. Epub 2014 Apr 24.
Benzo(a)pyrene (BaP) is a ubiquitous environmental pollutant. Studies have demonstrated it to be an endocrine-disrupting chemical that can cause adverse effects on the female reproductive system. However, the effect of BaP on early pregnancy has not been reported. We investigated the effect of BaP on endometrial receptivity and embryo implantation. Pregnant mice were dosed with BaP at 0.2, 2 and 20 mg/kg/day from day 1 (D1) to day 5 (D5) of gestation. Exposure to BaP impaired the morphology of the endometrium and decreased the number of implantation sites (p0.2=0.006, p2=0.167, p20=0.003). Levels of estrodiol (p<0.001, for three treatment group compare with control group) and progesterone-4 in plasma were elevated in BaP-treatment groups (p0.2<0.001, p2<0.001, p20=0.032). Expression of estrogen receptor-α was up-regulated (p0.2=0.002, p2=0.131, p20=0.024) whereas expression of the progesterone receptor was down-regulated (p0.2<0.001, p2=0.064, p20=0.021). Levels of receptivity-related genes HoxA10 (p0.2<0.001, p2=0.135, p20<0.001) and E-cadherin (p0.2=0.002, p2=0.624, p20=0.137) were changed by BaP. These results revealed that BaP can disrupt the balance of estrogen and progesterone, influence expression of their receptors and downstream related genes, lead to changes in endometrium receptivity, and reduce of the number of implantation sites.
苯并(a)芘（BaP）是一种普遍存在的环境污染物。研究表明它是一种内分泌干扰化学物质，可对女性生殖系统产生不良影响。然而，BaP对早期妊娠的影响尚未见报道。我们研究了BaP对子宫内膜容受性和胚胎着床的影响。妊娠小鼠从妊娠第1天（D1）至第5天（D5）每天给予0.2、2和20 mg/kg的BaP。暴露于BaP会损害子宫内膜形态并减少着床部位数量（p0.2 = 0.006，p2 = 0.167，p20 = 0.003）。BaP处理组血浆中雌二醇水平（三个处理组与对照组比较，p<0.001）和孕酮-4水平升高（p0.2<0.001，p2<0.001，p20 = 0.032）。雌激素受体-α的表达上调（p0.2 = 0.002，p2 = 0.131，p20 = 0.024），而孕酮受体的表达下调（p0.2<0.001，p2 = 0.064，p20 = 0.021）。BaP改变了容受性相关基因HoxA10（p0.2<0.001，p2 = 0.135，p20<0.001）和E-钙黏蛋白（p0.2 = 0.002，p2 = 0.624，p20 = 0.137）的水平。这些结果表明，BaP可破坏雌激素和孕酮的平衡，影响其受体及下游相关基因的表达，导致子宫内膜容受性改变，并减少着床部位数量。
