Zhou Jianhong, Qu Chengquan, Sun Qi, Wu Lisha, Liu Yanglu, Yang Zhirong, Zhang Jie
College of Life Sciences, Key Laboratory of Bio-resource and Bio-control, Sichuan University, Chengdu, Sichuan 610064, PR China.
College of Life Sciences, Key Laboratory of Bio-resource and Bio-control, Sichuan University, Chengdu, Sichuan 610064, PR China.
Chem Biol Interact. 2014 Aug 5;219:57-63. doi: 10.1016/j.cbi.2014.05.009. Epub 2014 May 28.
Sophoricoside (SOPH) is an isoflavone glycoside isolated from the fruits of Sophora japonica. Since its first isolation in 1961, there are rare findings about the effects of SOPH on reproductive system. In the present study, the pregnant mice administrated by different doses of SOPH were used to explore the effect of SOPH on embryo implantation, especially on the endometrial receptivity. The statistical results showed that the number of implanted embryos was gradually declining along the increasing dose of SOPH. When the administrated dose of SOPH was 600 mg/kg per day, great changes were observed in the exposed uterine morphology and up-regulated progesterone receptor (PR) and down-regulated estrogen receptor α (ERα), E-cadherin, matrix metalloproteinase-2 (MMP-2) and integrin β3 were also found in SOPH-exposed uterine. These findings demonstrated that SOPH exposure reduced the number of implanted embryos in a dose-dependent manner and failed the embryo implantation through altering the morphology of uterine and compromising the endometrial receptivity.
槐角苷（SOPH）是从槐的果实中分离得到的一种异黄酮苷。自1961年首次分离以来，关于SOPH对生殖系统影响的研究报道较少。在本研究中，通过给怀孕小鼠注射不同剂量的SOPH，探讨SOPH对胚胎着床的影响，尤其是对子宫内膜容受性的影响。统计结果显示，随着SOPH剂量的增加，着床胚胎数量逐渐减少。当SOPH给药剂量为每天600 mg/kg时，暴露子宫的形态发生了显著变化，孕激素受体（PR）上调，雌激素受体α（ERα）、E-钙黏蛋白、基质金属蛋白酶-2（MMP-2）和整合素β3下调。这些结果表明，SOPH暴露以剂量依赖的方式减少了着床胚胎的数量，并通过改变子宫形态和损害子宫内膜容受性而导致胚胎着床失败。
