Lorda-Diez Carlos I, García-Porrero Juan A, Hurlé Juan M, Montero Juan A
Departamento de Anatomía y Biología Celular and IFIMAV, Universidad de Cantabria, Santander 39011, Spain.
Departamento de Anatomía y Biología Celular and IFIMAV, Universidad de Cantabria, Santander 39011, Spain.
Gene Expr Patterns. 2014 May;15(1):52-60. doi: 10.1016/j.gep.2014.04.003. Epub 2014 Apr 24.
Tendons and cartilages are connective tissues of essential importance in the musculoskeletal system. During digit development, cartilage and tendon primordia develop from the undifferentiated mesenchymal cells originated in the lateral plate mesoderm. The specification of these tissues begins with the establishment of cellular aggregates, which prefigure the tendons and phalanges. Transforming growth factor beta proteins (TGFβs) are the inductive signals responsible not only for the initiation of chondrogenesis and tenogenesis during digit formation, but also for joint specification. An early role of this family of secreted proteins during these processes is to promote mesenchymal cell precursors condensation. Here we show that Decorin presents an overlapping pattern of expression with TGFβ2 in joint and tendon blastemas of the embryonic digits. Furthermore, Decorin expression is induced by TGFβ signaling, and DECORIN promotes aggregation of digit mesenchymal cell precursors. In addition, we provide gene expression studies suggesting that Cadherin-11 may function as an effector of Decorin in this experimental model.
肌腱和软骨是肌肉骨骼系统中至关重要的结缔组织。在手指发育过程中，软骨和肌腱原基由起源于侧板中胚层的未分化间充质细胞发育而来。这些组织的特化始于细胞聚集体的形成，这些聚集体预示着肌腱和指骨的形成。转化生长因子β蛋白（TGFβs）是诱导信号，不仅负责手指形成过程中软骨生成和肌腱生成的起始，还负责关节特化。这个分泌蛋白家族在这些过程中的早期作用是促进间充质细胞前体的凝聚。在这里，我们表明核心蛋白聚糖在胚胎手指的关节和肌腱芽中呈现出与TGFβ2重叠的表达模式。此外，核心蛋白聚糖的表达由TGFβ信号诱导，并且核心蛋白聚糖促进手指间充质细胞前体的聚集。此外，我们提供的基因表达研究表明，在这个实验模型中，钙黏蛋白-11可能作为核心蛋白聚糖的效应器发挥作用。
