School of Marine Science and Technology, Huaihai Institute of Technology, 59 Cangwu Road, Xinpu 222005, China.
Int J Biol Macromol. 2014 Jul;68:18-20. doi: 10.1016/j.ijbiomac.2014.04.020. Epub 2014 Apr 24.
In this study, investigated was the effect of the porphyra yezoensis polysaccharides (PPs) on the denaturation of bighead carp (Aristichthys nobilis) myofibrillar protein (Mf) during frozen storage at -18°C for 90d. The PPs (2.5%, 5%, and 7.5%, respectively) was added to 100g of Mf. The changes in the Ca-adenylpyrophosphatase (ATPase) activity and unfrozen water content in Mf were examined to evaluate he denaturation of Mf during frozen storage. Ca-ATPase activity decreased gradually during frozen storage at -18°C upon addition of PPs. By contrast, Ca-ATPase activity in the control group dropped drastically during the first 45d of storage and then further decreased gradually for up to 90d of storage, indicating a biphasic denaturation pattern. PPs addition significantly increased sulfhydryl contents in the Mf of the treatment groups compared with that of the control group (p<0.05) during frozen storage at -18°C.
在这项研究中，研究了紫菜多糖（PPs）对-18°C 冷冻储存 90d 期间大头鱼（Aristichthys nobilis）肌原纤维蛋白（Mf）变性的影响。分别向 100g 的 Mf 中添加 2.5%、5%和 7.5%的 PPs。通过检测 Ca-腺嘌呤核苷三磷酸酶（ATPase）活性和 Mf 中未冻结水的含量来评估 Mf 在冷冻储存过程中的变性情况。在-18°C 下添加 PPs 时，Ca-ATPase 活性在冷冻储存过程中逐渐下降。相比之下，对照组的 Ca-ATPase 活性在储存的前 45d 急剧下降，然后在 90d 的储存期内进一步逐渐下降，表明存在两相变性模式。在-18°C 下冷冻储存时，与对照组相比，添加 PPs 可显著增加处理组 Mf 中的巯基含量（p<0.05）。
