Adamczyk-Engelmann P, Bader H
University Ulm, FRG.
Agents Actions. 1989 Aug;28(1-2):34-8. doi: 10.1007/BF02022977.
Histamine release induced by compound 48/80 from rat mast cells is not dependent on extracellular Ca2+. Preincubation of mast cells with trypsin has only little effects on histamine release induced by this polycation. This work also demonstrates that histamine release induced by compound 48/80 and its analogues in the absence of extracellular Ca2+ depends on membrane bound sialic acid of the mast cell. Neuraminidase treatment of the cells in the presence of extracellular Ca2+ leads to histamine liberation. These findings suggest that sialic acid residues of the mast cell membrane constitute the site at which polycations exert their stimulatory actions of histamine liberation.
化合物48/80诱导大鼠肥大细胞释放组胺不依赖于细胞外钙离子。用胰蛋白酶预孵育肥大细胞对这种聚阳离子诱导的组胺释放影响很小。这项研究还表明，在没有细胞外钙离子的情况下，化合物48/80及其类似物诱导的组胺释放依赖于肥大细胞膜结合的唾液酸。在细胞外钙离子存在的情况下用神经氨酸酶处理细胞会导致组胺释放。这些发现表明，肥大细胞膜上的唾液酸残基构成了聚阳离子发挥其组胺释放刺激作用的位点。
