Jensen C, Norn S, Skov P S, Dahl B T, Thastrup O, Leon A, Svendsen U G
Agents Actions. 1987 Apr;20(3-4):161-4. doi: 10.1007/BF02074657.
The influence of the cell membrane content of sialic acid on basophil histamine release was examined in vitro in allergic patients and normal controls. Enzymatical removal of sialic acid enhanced histamine release induced by allergen and anti-IgE, whereas an increase in membrane sialic acid content by insertion of sialic acid containing gangliosides into the membrane inhibited the mediator release. The reduction in membrane sialic acid content abolished the inhibitory capacity of the calcium channel antagonist nimodipine, whereas the inhibition produced by verapamil and lanthanum was not affected. This difference, together with the previous finding that alterations in membrane sialic acid content is reflected in the cell sensitivity to extracellular calcium, suggest an interaction between membrane sialic acid and the calcium channels involved in basophil histamine release.
在体外研究了过敏性患者和正常对照者中细胞膜唾液酸含量对嗜碱性粒细胞组胺释放的影响。酶法去除唾液酸可增强变应原和抗IgE诱导的组胺释放，而通过将含唾液酸的神经节苷脂插入膜中增加膜唾液酸含量则抑制介质释放。膜唾液酸含量的降低消除了钙通道拮抗剂尼莫地平的抑制能力，而维拉帕米和镧产生的抑制作用不受影响。这种差异，连同先前发现的膜唾液酸含量的改变反映在细胞对细胞外钙的敏感性上，提示膜唾液酸与参与嗜碱性粒细胞组胺释放的钙通道之间存在相互作用。
