Wang Zhang-Xun, Zhou Xia-Zhi, Meng Hui-Min, Liu Yu-Jun, Zhou Quan, Huang Bo
Anhui Provincial Key Laboratory of Microbial Pest Control, Anhui Agricultural University, Hefei, 230036, China.
Appl Microbiol Biotechnol. 2014 Jun;98(12):5589-97. doi: 10.1007/s00253-014-5763-y. Epub 2014 Apr 26.
The entomopathogenic fungus Metarhizium anisopliae is widely used for biological control of a variety of insect pests. The effectiveness of the microbial pest control agent, however, is limited by poor thermotolerance. The molecular mechanism underlying the response to heat stress in the conidia of entomopathogenic fungi remains unclear. Here, we conducted high-throughput RNA-Seq to analyze the differential gene expression between control and heat treated conidia of M. anisopliae at the transcriptome level. RNA-Seq analysis generated 6,284,262 and 5,826,934 clean reads in the control and heat treated groups, respectively. A total of 2,722 up-regulated and 788 down-regulated genes, with a cutoff of twofold change, were identified by expression analysis. Among these differentially expressed genes, many were related to metabolic processes, biological regulation, cellular processes and response to stimuli. The majority of genes involved in endocytic pathways, proteosome pathways and regulation of autophagy were up-regulated, while most genes involved in the ribosome pathway were down-regulated. These results suggest that these differentially expressed genes may be involved in the heat stress response in conidia. As expected, significant changes in expression levels of genes encoding heat shock proteins and proteins involved in trehalose accumulation were observed in conditions of heat stress. These results expand our understanding of the molecular mechanisms of the heat stress response of conidia and provide a foundation for future investigations.
昆虫病原真菌绿僵菌被广泛用于多种害虫的生物防治。然而，这种微生物害虫防治剂的有效性受到耐热性差的限制。昆虫病原真菌分生孢子对热应激反应的分子机制仍不清楚。在此，我们进行了高通量RNA测序，以在转录组水平分析绿僵菌对照和热处理分生孢子之间的差异基因表达。RNA测序分析在对照组和热处理组中分别产生了6,284,262和5,826,934条clean reads。通过表达分析，共鉴定出2,722个上调基因和788个下调基因，变化阈值为两倍。在这些差异表达基因中，许多与代谢过程、生物调节、细胞过程和对刺激的反应有关。参与内吞途径、蛋白酶体途径和自噬调节的大多数基因上调，而参与核糖体途径的大多数基因下调。这些结果表明，这些差异表达基因可能参与分生孢子的热应激反应。正如预期的那样，在热应激条件下观察到编码热休克蛋白和参与海藻糖积累的蛋白质的基因表达水平发生了显著变化。这些结果扩展了我们对分生孢子热应激反应分子机制的理解，并为未来的研究提供了基础。
