McCauliffe D P, Lux F A, Lieu T S, Sanz I, Hanke J, Newkirk M M, Siciliano M J, Sontheimer R D, Capra J D
Department of Dermatology, U.T. Southwestern Medical Center, Dallas, Texas.
J Autoimmun. 1989 Aug;2(4):375-81. doi: 10.1016/0896-8411(89)90166-2.
Ro/SS-A is a soluble cellular protein to which antibodies are frequently directed in patients with Sjögren's syndrome (SS) and systemic lupus erythematosus (SLE)-related disorders. The pathogenic significance of these antibodies is uncertain. Our effort to further characterize the immunological and structural nature of Ro/SS-A suggests that the native human Ro/SS-A molecule is not glycosylated and consists of two disulfide linked domains. The amino terminus of the smaller domain contains a major epitope reactive with anti-Ro/SS-A sera. We have isolated and characterized a complementary DNA (cDNA) clone which encodes the Ro/SS-A polypeptide. The Ro/SS-A gene does not appear to be highly polymorphic and exists as a single copy on the short arm of chromosome 19. The isolation of the Ro/SS-A cDNA should allow for a more complete characterization of Ro/SS-A epitopes which should help further elucidate the importance of Ro/SS-A antibodies in the pathogenesis of disease.
Ro/SS-A是一种可溶性细胞蛋白，干燥综合征（SS）和系统性红斑狼疮（SLE）相关疾病患者体内常出现针对该蛋白的抗体。这些抗体的致病意义尚不确定。我们进一步表征Ro/SS-A免疫和结构性质的研究表明，天然人Ro/SS-A分子未进行糖基化，由两个通过二硫键连接的结构域组成。较小结构域的氨基末端含有与抗Ro/SS-A血清反应的主要表位。我们已分离并表征了一个编码Ro/SS-A多肽的互补DNA（cDNA）克隆。Ro/SS-A基因似乎并非高度多态性，在19号染色体短臂上以单拷贝形式存在。Ro/SS-A cDNA的分离应能更全面地表征Ro/SS-A表位，这将有助于进一步阐明Ro/SS-A抗体在疾病发病机制中的重要性。
