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提取和加工方法对紫菜(条斑紫菜)抗氧化化合物含量及自由基清除活性的影响

Effects of Extraction and Processing Methods on Antioxidant Compound Contents and Radical Scavenging Activities of Laver (Porphyra tenera).

作者信息

Hwang Eun-Sun, Thi Nhuan Do

机构信息

Department of Nutrition and Culinary Science, Hankyong National University, Gyeonggi 456-749, Korea ; Korean Foods Global Center, Hankyong National University, Gyeonggi 456-749, Korea.

Department of Nutrition and Culinary Science, Hankyong National University, Gyeonggi 456-749, Korea.

出版信息

Prev Nutr Food Sci. 2014 Jan;19(1):40-8. doi: 10.3746/pnf.2014.19.1.040.

Abstract

Laver is one of the most consumed edible red algae seaweeds in the genus Porphyra. Laver is primarily prepared in the form of dried, roasted, and seasoned products. We investigated the total polyphenol and flavonoid contents of laver products, and evaluated the in vitro antioxidant properties of solvent extracts from commercially processed laver products. Significant differences in the concentration of phenolic compounds were found among differently processed laver. The total phenolic content for laver extracts ranged from 10.81 mg gallic acid equivalent (GAE)/g extract to 32.14 mg GAE/g extract, depending on extraction solvent and temperature. Laver extracts contained very few flavonoids (0.55 mg catechin equivalent/g extracts to 1.75 mg catechin equivalent/g extracts). 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS), hydroxyl radical, and superoxide anion scavenging assays were used to determine the radical scavenging capacities of laver extracts. These assays revealed that the processing method and extraction condition affected the antioxidant potentials of laver. Antioxidant activity of dried laver, roasted laver, and seasoned laver increased in a concentration-dependent manner (100~1,000 μg/mL). The radical scavenging activities of 37°C and 100°C water extracts were lower than that of a 37°C 70% ethanol extract. The highest radical scavenging capacity was observed in the 37°C 70% ethanol extracts of dried laver, roasted laver, and seasoned laver. Overall, these results support that notion that laver contains bioactive compounds, such as polyphenols and flavonoids, which may have a positive effect on health.

摘要

紫菜是紫菜属中食用量最大的可食用红藻海草之一。紫菜主要制成干制品、烤制产品和调味产品。我们研究了紫菜产品中的总多酚和黄酮含量,并评估了市售加工紫菜产品溶剂提取物的体外抗氧化性能。不同加工方式的紫菜中酚类化合物的浓度存在显著差异。根据提取溶剂和温度的不同,紫菜提取物的总酚含量在10.81毫克没食子酸当量(GAE)/克提取物至32.14毫克GAE/克提取物之间。紫菜提取物中的黄酮类化合物含量极少(0.55毫克儿茶素当量/克提取物至1.75毫克儿茶素当量/克提取物)。采用2,2-二苯基-1-苦基肼(DPPH)、2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)、羟基自由基和超氧阴离子清除试验来测定紫菜提取物的自由基清除能力。这些试验表明,加工方法和提取条件会影响紫菜的抗氧化能力。干紫菜、烤紫菜和调味紫菜的抗氧化活性呈浓度依赖性增加(100~1000微克/毫升)。37℃和100℃水提取物的自由基清除活性低于37℃ 70%乙醇提取物。在干紫菜、烤紫菜和调味紫菜的37℃ 70%乙醇提取物中观察到最高的自由基清除能力。总体而言,这些结果支持了紫菜含有生物活性化合物(如多酚和黄酮类化合物)的观点,这些化合物可能对健康有积极影响。

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