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十八种植物不同溶剂提取物的抗氧化化学成分及抗氧化性能探究

Exploration of the Antioxidant Chemical Constituents and Antioxidant Performance of Various Solvent Extracts of Eighteen Plants.

作者信息

Seleshe Semeneh, Ameer Ammara, Kang Suk Nam

机构信息

Department of Animal Resource, Daegu University, Gyeongbuk 38453, Korea.

出版信息

Prev Nutr Food Sci. 2022 Jun 30;27(2):212-222. doi: 10.3746/pnf.2022.27.2.212.

Abstract

This study examined the antioxidant chemical constituents (total phenolic and total flavonoid contents) and antioxidant activities [1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging, and ferric reducing antioxidant power (FRAP)] of different solvent extracts of eighteen functional plants. The active components of the target plants were extracted using four different solvents (methanol, ethanol, chloroform, and water). leaf exhibited a higher total phenolic content (chloroform and water extracts), total flavonoid content (all solvent extracts), DPPH radical scavenging activity (methanol, ethanol, and water extracts), ABTS radical scavenging activity (water extract), and FRAP radical scavenging activity (water extract) (in all solvent extracts). Similarly, root was another excellent and competent extract with a high total flavonoid content (in all four solvent extracts), DPPH and ABTS radical scavenging activity (methanol, ethanol, and water extracts), and FRAP activity (methanol, ethanol, and water extracts) (in all solvent extracts). fruit had the highest total phenolic content (methanol, ethanol, and water extracts), DPPH and ABTS radical scavenging activate (in all solvent extracts), and FRAP in its water extract were the two most effective functional plants. Based on the abundance of antioxidant chemical constituents and the most potent antioxidant activity demonstrated in this study, extracts from leaf, root, and fruit appear to be promising candidates to meet the current demand for natural preservatives in food and pharmaceutical industries.

摘要

本研究检测了18种功能性植物不同溶剂提取物的抗氧化化学成分(总酚和总黄酮含量)及抗氧化活性[1,1-二苯基-2-苦基肼(DPPH)和2,2'-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)(ABTS)自由基清除能力以及铁离子还原抗氧化能力(FRAP)]。使用四种不同溶剂(甲醇、乙醇、氯仿和水)提取目标植物的活性成分。叶片在所有溶剂提取物中表现出较高的总酚含量(氯仿和水提取物)、总黄酮含量(所有溶剂提取物)、DPPH自由基清除活性(甲醇、乙醇和水提取物)、ABTS自由基清除活性(水提取物)以及FRAP自由基清除活性(水提取物)。同样,根部也是另一种优良且有效的提取物,在所有四种溶剂提取物中均具有较高的总黄酮含量、DPPH和ABTS自由基清除活性(甲醇、乙醇和水提取物)以及FRAP活性(甲醇、乙醇和水提取物)。果实的总酚含量最高(甲醇、乙醇和水提取物)、DPPH和ABTS自由基清除活性(所有溶剂提取物),其水提取物中的FRAP是两种最有效的功能性植物。基于本研究中抗氧化化学成分的丰富度和最强大的抗氧化活性,叶片、根部和果实的提取物似乎有望满足食品和制药行业目前对天然防腐剂的需求。

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