Department of Bioscience, Zoophysiology, Aarhus University, C.F.Møllers Allé 3, DK-8000, Aarhus C, Denmark.
J Fish Biol. 2014 May;84(5):1326-39. doi: 10.1111/jfb.12356.
Using rainbow trout Oncorhynchus mykiss, the present study demonstrated that: (1) calcium (Ca) increased the range of copper (Cu) concentrations that O. mykiss avoided; (2) Ca conserved the maintenance of pre-exposure swimming activity during inescapable acute (10 min) Cu exposure. Data showed that when presented with a choice of Cu-contaminated water (ranging from 0 to 454 µg Cu l⁻¹ ) and uncontaminated water in a choice tank, O. mykiss acclimated and tested at low Ca concentration (3 mg Ca l⁻¹ avoided the 10 µg Cu l⁻¹ only. By contrast, O. mykiss acclimated and tested at high Ca concentration (158 mg Ca l⁻¹) avoided all the Cu concentrations ≥37 µg⁻¹. The Cu avoidance was connected with increased spontaneous swimming speed in the Cu-contaminated water. When subjected to inescapable Cu exposure (35 µg Cu l⁻¹), O. mykiss acclimated and tested at low Ca concentration reduced their spontaneous swimming speed, whereas no response was observed in O. mykiss acclimated and tested at high Ca concentration. Collectively, the data support the conclusion that in O. mykiss the behavioural responses to acute Cu exposure are Ca-dependent.
利用虹鳟鱼 Oncorhynchus mykiss，本研究表明：(1) 钙 (Ca) 增加了虹鳟鱼回避的铜 (Cu) 浓度范围；(2) Ca 可维持在不可避免的急性 (10 min) Cu 暴露期间，预暴露游泳活动的维持。研究数据表明，当在选择箱中面临 Cu 污染水（0 至 454 µg Cu l⁻¹）和未污染水的选择时，适应低 Ca 浓度（3 mg Ca l⁻¹）的虹鳟鱼回避了 10 µg Cu l⁻¹。相比之下，适应和测试于高 Ca 浓度（158 mg Ca l⁻¹）的虹鳟鱼回避了所有 Cu 浓度≥37 µg⁻¹。Cu 的回避与 Cu 污染水中自发游泳速度的增加有关。当面临不可避免的 Cu 暴露（35 µg Cu l⁻¹）时，适应和测试于低 Ca 浓度的虹鳟鱼降低了它们的自发游泳速度，而适应和测试于高 Ca 浓度的虹鳟鱼没有观察到这种反应。综合数据表明，在虹鳟鱼中，对急性 Cu 暴露的行为反应依赖于 Ca。
