Blood Systems Research Institute, San Francisco, CA 94118, USA; Department of Laboratory Medicine, University of California at San Francisco, San Francisco, CA 94118, USA.
Division of Molecular Virology, Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.
J Clin Virol. 2014 Jun;60(2):161-4. doi: 10.1016/j.jcv.2014.03.024. Epub 2014 Apr 13.
A significant fraction of cases of diarrhea, a leading cause of childhood mortality worldwide, remain unexplained.
To identify viruses in unexplained cases of diarrhea using an unbiased metagenomics approach.
Viral nucleic acids were enriched from the feces from 48 cases of unexplained diarrhea from Burkina Faso, sequenced, and compared against all known viral genomes.
The full genome of a highly divergent astrovirus was sequenced in a sample co-infected with parechovirus 1. RT-PCR identified a single astrovirus infection in these 48 patients indicating a low prevalence. Human astrovirus-BF34 was most closely related to mamastrovirus species 8 and 9 also found in human with which it shared 62%, 74%, and 57% amino acid identities over its protease, RNA dependent RNA polymerase and capsid proteins, respectively.
Burkina Faso astrovirus is proposed as prototype for a novel species in the genus Mamastrovirus, here tentatively called Mamastrovirus 20, representing the fifth human astrovirus species.
腹泻是全球儿童死亡的主要原因之一，其中很大一部分病例的病因仍不清楚。
采用无偏倚的宏基因组学方法鉴定不明原因腹泻病例中的病毒。
从布基纳法索 48 例不明原因腹泻的粪便中富集病毒核酸，进行测序，并与所有已知病毒基因组进行比较。
在与 parechovirus 1 共同感染的样本中，测序了一种高度分化的星状病毒的全基因组。RT-PCR 在这 48 例患者中鉴定出单一的星状病毒感染，表明其患病率较低。人星状病毒-BF34 与在人类中发现的 mamastrovirus 种 8 和 9 最为密切相关，其在蛋白酶、RNA 依赖性 RNA 聚合酶和衣壳蛋白上的氨基酸同一性分别为 62%、74%和 57%。
布基纳法索星状病毒被提议为 Mamastrovirus 属中新种的原型，暂命名为 Mamastrovirus 20，代表第五种人类星状病毒。
