Stepanova N V, Iaremko E E, Petrenko V V
Lab Delo. 1989(7):28-30.
Serotonin and histamine are important components of neurohumoral regulation. The biologic and fluorometric methods currently used for their measurements are labor-consuming and involve the use of unavailable reagents and expensive equipment. The authors suggest a sensitive simplified technique for serotonin and histamine measurements in a small volume of the biologic material (tissue and blood). The procedure is as follows: serotonin and histamine are isolated by thin-layer chromatography, the obtained samples are treated with alloxan in dimethyl formamide medium, and the stained solutions are examined in spectrophotometer. The suggested method is highly specific and sensitive, simple, makes use of Soviet equipment and of available reagents, this recommending it for clinical biochemical, as well as for experimental studies.
血清素和组胺是神经体液调节的重要组成部分。目前用于测量它们的生物学和荧光测定方法既耗时，又需要使用难以获得的试剂和昂贵的设备。作者提出了一种灵敏、简化的技术，用于在少量生物材料（组织和血液）中测量血清素和组胺。操作步骤如下：通过薄层色谱法分离血清素和组胺，将所得样品在二甲基甲酰胺介质中用四氧嘧啶处理，然后在分光光度计中检测染色溶液。所建议的方法具有高度特异性和灵敏性，操作简单，使用苏联设备和现有试剂，因此推荐用于临床生化以及实验研究。
