Makarova G I, Alekseenkova L P
Lab Delo. 1989(8):72-3.
Use of twice-distilled water or addition of normal equine serum, preheated at 56 degrees C for 30 min and adsorbed with an adsorbent from the diagnostic kit, to normal saline (1 ml of serum per 100 ml of the saline) helps eliminate spontaneous agglutination of the tested diagnosticum during the detection of HBsAg with the use of reverse passive hemagglutination test, when the experiments are carried out in areas with high concentrations of salts and organic substances in the water.
在水的盐和有机物浓度较高的地区进行实验时,使用双蒸水或将在56℃预热30分钟并用诊断试剂盒中的吸附剂吸附过的正常马血清加入生理盐水(每100ml生理盐水加入1ml血清),有助于在使用反向被动血凝试验检测HBsAg时消除被测诊断试剂的自发凝集。