三种被动血凝技术检测乙型肝炎表面抗原的比较评价（作者译）

[Comparative evaluation of three techniques of passive haemoagglutination for the determination of HBsAg (author's transl)].

作者信息

Aceti A, Vullo V, Ferone U, Delia S

出版信息

Quad Sclavo Diagn. 1978 Dec;14(4):451-7.

Abstract

The AA. studied the reaction of indirect haemoagglutination for the determination of HBsAg in 157 sera from patients with viral hepatitis, with 3 differents commercial tests based on the agglutination by HBsAg, respectively, of sheep, turkey and human erythrocytes sensitized with antibody to HBsAg. All three techniques showed a high sensibility comparable to that of the RIA. Regarding specificity the best results were obtained with the turkey and human erythrocytes, while the sheep erythrocytes because of the presence of some aspecific positivity, need a confirmatory test.

摘要

作者研究了间接血凝反应，以检测157份病毒性肝炎患者血清中的乙肝表面抗原（HBsAg），分别采用了三种不同的基于乙肝表面抗原致敏绵羊、火鸡和人红细胞凝集反应的商业检测方法。所有这三种技术均显示出与放射免疫分析（RIA）相当的高灵敏度。在特异性方面，火鸡和人红细胞检测获得了最佳结果，而绵羊红细胞由于存在一些非特异性阳性反应，需要进行确证试验。

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