Ma Xue, Zhou Xin-Yu, Qiang Qian-Qian, Zhang Zhi-Qi
Key Laboratory of the Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, China.
J Sep Sci. 2014 Jul;37(14):1834-41. doi: 10.1002/jssc.201400070. Epub 2014 May 28.
An aqueous solution of polyethylene glycol (PEG) as a green solvent was employed for the first time to develop the ultrasound-assisted extraction of proanthocyanidins (PA) and chlorogenic acid (CA) from almond skin. The optimized extraction parameters were determined based on response surface methodology, and corresponded to an ultrasound power of 120 W, a liquid-to-solid ratio of 20:1 (mL/g), and a PEG concentration of 50% (v/v). Under these optimized conditions, the extraction yields of PAs and CA from almond skin were 32.68 ± 0.22 and 16.01 ± 0.19 mg/g, respectively. Compared with organic solvent extraction, PEG solution extraction produced higher yields. Different macroporous resins were compared for their performance in purifying PAs and CA from almond skin extract. Static adsorption/desorption experimental results demonstrated that AB-8 resin exhibits excellent purification performance at pH 4. Under the optimized dynamic adsorption/desorption conditions on the AB-8 column, the total recovery of purification for PAs and CA was 80.67%. The total content of PAs and CA in the preliminarily purified extract was 89.17% (with respective contents of 60.90 and 28.27%).
首次采用聚乙二醇（PEG）水溶液作为绿色溶剂，用于超声辅助从杏仁皮中提取原花青素（PA）和绿原酸（CA）。基于响应面法确定了优化的提取参数，其对应于120W的超声功率、20:1（mL/g）的液固比以及50%（v/v）的PEG浓度。在这些优化条件下，从杏仁皮中提取PA和CA的产率分别为32.68±0.22和16.01±0.19mg/g。与有机溶剂提取相比，PEG溶液提取产率更高。比较了不同大孔树脂从杏仁皮提取物中纯化PA和CA的性能。静态吸附/解吸实验结果表明，AB-8树脂在pH 4时表现出优异的纯化性能。在AB-8柱上优化的动态吸附/解吸条件下，PA和CA的纯化总回收率为80.67%。初步纯化提取物中PA和CA的总含量为89.17%（各自含量为60.90和28.27%）。
