Alegre Melissa M, Weyant Michael J, Bennett Daine T, Yu Jessica A, Ramsden Madison K, Elnaggar Atif, Robison Richard A, O'Neill Kim L
Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, 84602, U.S.A.
Anticancer Res. 2014 May;34(5):2145-51.
Thymidine kinase 1 (TK1) is a biomarker elevated in several malignancies, including lung cancer. Up-regulation of TK1 is an early event in carcinogenesis and therefore a target for early cancer detection. We have developed a novel Enzyme Linked Immunosorbent Assay (ELISA) to detect TK1 in serum.
Forty patients with pulmonary nodules and 18 healthy individuals had their serum collected prior to surgery. All samples were analyzed using a radioassay and ELISA.
TK1 was significantly elevated in all lung cancer samples. Patients with stage I (n=16) and stage II (n=17) disease had significantly higher TK1 levels than controls. The area under the curve was 0.792, using 4.9 nM TK1 as cut-off, for early-stage lung cancer. The sensitivity and specificity were 75.0 and 83.3, respectively. TK1 concentration was a more sensitive and accurate indicator of lung cancer than TK1 activity.
TK1 is significantly elevated in serum from patients with stage I and stage II lung cancer as measured using the established ELISA. This novel TK1 ELISA is both sensitive and specific for the detection of early-stage and advanced lung cancer, and therefore may be an important tool in the management of this disease.
胸苷激酶1（TK1）是一种在包括肺癌在内的多种恶性肿瘤中升高的生物标志物。TK1的上调是致癌过程中的早期事件，因此是早期癌症检测的靶点。我们开发了一种新型酶联免疫吸附测定法（ELISA）来检测血清中的TK1。
40例肺结节患者和18名健康个体在手术前采集血清。所有样本均采用放射免疫分析法和ELISA进行分析。
所有肺癌样本中的TK1均显著升高。I期（n = 16）和II期（n = 17）疾病患者的TK1水平显著高于对照组。以4.9 nM TK1为临界值，早期肺癌的曲线下面积为0.792。敏感性和特异性分别为75.0和83.3。TK1浓度比TK1活性更敏感、准确地指示肺癌。
使用已建立的ELISA法检测，I期和II期肺癌患者血清中的TK1显著升高。这种新型TK1 ELISA对早期和晚期肺癌的检测既敏感又特异，因此可能是该疾病管理中的重要工具。
