AroCell AB, Virdings allé 32B, SE-754 50 Uppsala, Sweden.
Diagnostics Development a P&M Venge AB Company, Uppsala, Sweden.
Biotechniques. 2020 Jun;68(6):334-341. doi: 10.2144/btn-2019-0148. Epub 2020 Apr 27.
Thymidine kinase 1 (TK1) is an enzyme involved in DNA precursor synthesis that has been used as a biomarker for prognosis and monitoring of different malignancies. In this study, we compared two immunoassays for measuring TK1 protein concentrations: the TK 210 ELISA (AroCell AB) and TK1 ELISA from Abcam. Overall, the TK 210 ELISA showed higher sensitivity than the Abcam TK1 ELISA for differentiating hematological malignancies (sensitivity of 0.77 vs 0.45) as well as for distinguishing sera of patients with solid tumors from those of apparently healthy individuals (0.61 vs 0.20). There was no significant difference in the TK1 protein levels determined with the TK 210 ELISA between different age groups from apparently healthy individuals. These results strongly indicate that the AroCell TK 210 ELISA is accurate and sensitive enough to be a valuable tool in cancer management.
胸苷激酶 1（TK1）是一种参与 DNA 前体合成的酶，已被用作不同恶性肿瘤的预后和监测生物标志物。在这项研究中，我们比较了两种用于测量 TK1 蛋白浓度的免疫分析试剂盒：AroCell AB 的 TK210 ELISA 和 Abcam 的 TK1 ELISA。总体而言，与 Abcam TK1 ELISA 相比，TK210 ELISA 用于区分血液系统恶性肿瘤的灵敏度更高（灵敏度为 0.77 比 0.45），用于区分实体瘤患者和健康个体的血清的灵敏度也更高（0.61 比 0.20）。来自健康个体的不同年龄组的 TK210 ELISA 测定的 TK1 蛋白水平没有显著差异。这些结果强烈表明，AroCell TK210 ELISA 足够准确和灵敏，是癌症管理的有价值工具。
