Fritsch H
Institute of Anatomy, Medical University of Lübeck, Federal Republic of Germany.
Stain Technol. 1989 Mar;64(2):75-9. doi: 10.3109/10520298909108049.
Sections of undecalcified human fetuses, fixed in formaldehyde, embedded in the epoxy resin Biodur E 12 and cut on a diamond-wire saw were stained according to a slight modification of the method described by Laczkó and Lévai. The sections were immersed in a methylene blue/azure II solution at 90 C for at least 3 min and counterstained with a basic fuchsin solution at the same temperature. Differential staining was as follows: bone stained pinkish; cartilage, violet; collagen fibers, blue-violet; elastic fibers, red and muscle fibers, green-blue. Most other tissues were stained blue-violet against the transparent background of the embedding epoxy resin. Thanks to the distinct and differential staining of each tissue, contrast is sufficient for black and white as well as for color photography.
将用甲醛固定、包埋于环氧树脂Biodur E 12中并用金刚石线锯切割的未脱钙人胎儿切片，按照Laczkó和Lévai所述方法的微小修改进行染色。切片在90℃下浸入亚甲蓝/天青II溶液中至少3分钟，然后在相同温度下用碱性品红溶液进行复染。鉴别染色如下：骨染成粉红色；软骨染成紫色；胶原纤维染成蓝紫色；弹性纤维染成红色；肌纤维染成绿蓝色。大多数其他组织在包埋环氧树脂的透明背景下染成蓝紫色。由于每种组织都有明显的鉴别染色，黑白摄影和彩色摄影的对比度都足够。
