Grill V, Zweyer M, Bareggi R, Martelli A M, Basa M, Narducci P
Department of Human Morphology, University of Trieste, Italy.
Biotech Histochem. 1995 Mar;70(2):75-80. doi: 10.3109/10520299509108321.
In this report we describe a simple and rapid staining technique for cartilage and bone embedded in Araldite. Semithin sections of embryonic vertebrae obtained from 15 to 17 day mouse fetuses were stained using an aqueous solution 0.25% with respect to methylene blue, 0.25% with respect to azure A, and 0.5% with respect to Na2 CO3, then counterstained with 1% aqueous pararosaniline chloride (MAP). Results were compared with toluidine blue stained sections. MAP permitted good discrimination of developmental stages of both cells and extracellular matrix within vertebral ossification centers during endochondral ossification. The technique is simple, rapid and applicable to plastic embedded sections, and can be used prior to ultrastructural examination.
在本报告中，我们描述了一种用于对包埋在环氧树脂中的软骨和骨进行简单快速染色的技术。使用相对于亚甲蓝为0.25%、相对于天青A为0.25%、相对于碳酸钠为0.5%的水溶液对从15至17日龄小鼠胎儿获取的胚胎椎骨半薄切片进行染色，然后用1%的对氯苯玫瑰苯胺水溶液（MAP）进行复染。将结果与甲苯胺蓝染色切片进行比较。MAP能够很好地区分软骨内成骨过程中椎骨骨化中心内细胞和细胞外基质的发育阶段。该技术简单、快速且适用于塑料包埋切片，可在超微结构检查之前使用。
