Tsubura A, Inui T, Senzaki H, Morii S, Dairkee S H
Department of Pathology, Kansai Medical University, Osaka, Japan.
Virchows Arch A Pathol Anat Histopathol. 1989;415(6):533-8. doi: 10.1007/BF00718646.
Using immunoperoxidase staining of monoclonal antibody 312C8-1 against 51,000 dalton human keratin polypeptide, immunolocalization was observed in frozen sections of normal tissue and mammary tumours of adult female mice and rats. In normal tissue, the epitope was recognized in myoepithelial cells of the mammary, sweat and salivary glands, and in basal and suprabasal cells of the epidermis. However, the antibody did not react with luminal epithelial cells of the above glands or with mesenchymal cells. In spontaneous mammary tumours of mice, marker-positive tumour cells were distributed only in the outer layer of adenocarcinoma Type A, while they were scattered in some foci of adenocarcinoma Type B, and encircled the epithelial foci of pregnancy dependent tumours (plaque). All layers of epidermoid structures in adenoacanthoma revealed positivity. In rat mammary tumours induced by local dusting with 7, 12-dimethylbenz(a)anthracene (DMBA) powder, the staining pattern of benign tumours was comparable to that of the normal mammary gland. But, in addition to basally situated cells, marker-positive tumour cells were found scattered in the foci of adenocarcinoma, and were not restricted to basal cells in squamous cell carcinoma. The marker was not found in sarcomatous tissue. This antibody can therefore also be applied to rodents, and the staining pattern can be used to identify the epithelial subclass specific marker in normal tissue and in mammary tumours.
利用针对51,000道尔顿人角蛋白多肽的单克隆抗体312C8 - 1进行免疫过氧化物酶染色，在成年雌性小鼠和大鼠的正常组织及乳腺肿瘤的冰冻切片中观察到免疫定位情况。在正常组织中，该表位在乳腺、汗腺和唾液腺的肌上皮细胞以及表皮的基底细胞和基底上层细胞中被识别。然而，该抗体不与上述腺体的腔上皮细胞或间充质细胞发生反应。在小鼠的自发性乳腺肿瘤中，标记阳性的肿瘤细胞仅分布在A型腺癌的外层，而在B型腺癌的一些病灶中呈散在分布，并环绕着妊娠依赖性肿瘤（斑块）的上皮病灶。腺棘皮瘤中所有表皮样结构层均呈阳性。在用7,12 - 二甲基苯并(a)蒽（DMBA）粉末局部涂抹诱导的大鼠乳腺肿瘤中，良性肿瘤的染色模式与正常乳腺组织相似。但是，除了位于基底的细胞外，标记阳性的肿瘤细胞还散在于腺癌病灶中，并且在鳞状细胞癌中不限于基底细胞。在肉瘤组织中未发现该标记物。因此，这种抗体也可应用于啮齿动物，其染色模式可用于识别正常组织和乳腺肿瘤中的上皮亚类特异性标记物。
