Griffey S M, Madewell B R, Dairkee S H, Hunt J E, Naydan D K, Higgins R J
Department of Pathology, School of Veterinary Medicine, University of California, Davis.
Vet Pathol. 1993 Mar;30(2):155-61. doi: 10.1177/030098589303000208.
Human basal epithelium (myoepithelium)-specific (312C8-1) and luminal epithelium-specific (13H5) cytokeratin antibodies were applied to frozen sections of normal canine mammary tissues (seven), benign adenomas and hyperplasias (five), mixed tumors (12), and adenocarcinomas (18) to determine if epithelial subsets could be discriminated by the use of an avidin biotin peroxidase complex immunohistochemical procedure. The 312C8-1 and 13H5 antibodies were consistently reactive with basal and luminal epithelium, respectively, in the normal mammary gland (7/7) and in benign adenomas and hyperplasias (5/5). Mixed mammary tumors had similar basal and luminal epithelial reactivity and also had proliferating spindle-shaped stromal cells that were reactive with 312C8-1 (10/12) and 13H5 (4/12). The adenocarcinomas were subclassified into basal, luminal, and basal/luminal on the basis of 312C8-1 reactivity (4/18), 13H5 reactivity (2/18), and dual reactivity with mutually exclusive anatomic distribution (11/18), respectively. Those tumors with dual immunoreactivity were indicative of noninvasive carcinomas. Dogs with neoplasms that were reactive with 312C8-1 and nonreactive with 13H5 had local recurrence or distant metastasis within 2 weeks to 6 months after diagnosis. Other antibodies used for comparison were pan cytokeratin AE1/AE3, actin HHF35, and vimentin. 312C8-1 and 13H5 antibodies are specific for canine mammary basal and luminal epithelium, respectively, and by employing these antibodies, the origin and differentiation of canine mammary neoplasms can be determined more accurately than on the basis of hematoxylin and eosin-stained tissue alone.
将人基底上皮（肌上皮）特异性（312C8 - 1）和腔上皮特异性（13H5）细胞角蛋白抗体应用于正常犬乳腺组织（7例）、良性腺瘤和增生（5例）、混合瘤（12例）及腺癌（18例）的冰冻切片，以确定是否可通过抗生物素蛋白 - 生物素过氧化物酶复合物免疫组织化学方法区分上皮亚群。在正常乳腺（7/7）以及良性腺瘤和增生（5/5）中，312C8 - 1和13H5抗体分别始终与基底上皮和腔上皮发生反应。混合性乳腺肿瘤具有相似的基底和腔上皮反应性，并且还具有增殖的梭形基质细胞，这些细胞与312C8 - 1（10/12）和13H5（4/12）发生反应。根据312C8 - 1反应性（4/18）、13H5反应性（2/18）以及具有相互排斥的解剖分布的双重反应性（11/18），将腺癌分为基底型、腔型和基底/腔型。那些具有双重免疫反应性的肿瘤提示为非浸润性癌。与312C8 - 1发生反应且与13H5不发生反应的肿瘤犬在诊断后2周内至6个月内出现局部复发或远处转移。用于比较的其他抗体包括泛细胞角蛋白AE1/AE3、肌动蛋白HHF35和波形蛋白。312C8 - 1和13H5抗体分别对犬乳腺基底上皮和腔上皮具有特异性，通过使用这些抗体，比仅基于苏木精和伊红染色的组织能更准确地确定犬乳腺肿瘤的起源和分化。
