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豆荚螟(Maruca testulalis,鳞翅目:草螟科:斑螟亚科)的完整线粒体基因组

The complete mitochondrial genome of the bean pod borer, Maruca testulalis (Lepidoptera: Crambidae: Spilomelinae).

作者信息

Zou Yuan, Ma Wenqi, Zhang Lifang, He Shilin, Zhang Xiuyue, Tao Zeng

机构信息

a Key Laboratory of Bio-resources and Eco-environment (Ministry of Education) , College of Life Sciences, Sichuan University , Chengdu , P.R. China and.

b Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife , College of Life Sciences, Sichuan University , Sichuan , P.R. China.

出版信息

Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(1):740-1. doi: 10.3109/19401736.2014.913167. Epub 2014 Apr 29.

Abstract

In this study, the complete mitochondrial genome of Maruca testulalis was determined (GenBank accession number KJ623250). It is 15,110 bp in length, and included 13 PCGs, 2 rRNA gens, 22 tRNA genes, and a A + T-rich region. Its gene order and orientation are identical to other crambid species. The AT-skew and GC-skew of the entire mitogenome are negative and the nucleotide composition is biased toward A + T nucleotides (80.6%). All PCGs begin with ATN codons, except for COI which is initiated by CGA. All the 22 tRAN genes can be fold into the typical clover-leaf secondary structure except for tRNA(ser)(AGN). The 335 bp long A + T-rich region is located between srRNA and tRNA(Met) and contains some common features of the other lepidopterans, including the motif ATAGT followed by 14 bp poly-T stretches and a microsatellite-like (AT)11 elements preceded by the ATTTA motif.

摘要

在本研究中,测定了豆野螟(Maruca testulalis)的完整线粒体基因组(GenBank登录号KJ623250)。其长度为15,110 bp,包括13个蛋白质编码基因(PCGs)、2个核糖体RNA基因、22个转运RNA基因和一个富含A + T的区域。其基因顺序和方向与其他草螟科物种相同。整个线粒体基因组的AT偏斜和GC偏斜均为负值,核苷酸组成偏向于A + T核苷酸(80.6%)。除了由CGA起始的细胞色素氧化酶亚基I(COI)外,所有PCGs均以ATN密码子起始。除了丝氨酸转运RNA(AGN)外,所有22个转运RNA基因均可折叠成典型的三叶草二级结构。富含A + T的335 bp区域位于小核糖体RNA和甲硫氨酸转运RNA(tRNA(Met))之间,包含其他鳞翅目昆虫的一些共同特征,包括基序ATAGT,其后跟着14 bp的多聚T序列,以及在ATTTA基序之前的类似微卫星的(AT)11元件。

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