Zou Yuan, Ma Wenqi, Zhang Lifang, He Shilin, Zhang Xiuyue, Tao Zeng
a Key Laboratory of Bio-resources and Eco-environment (Ministry of Education) , College of Life Sciences, Sichuan University , Chengdu , P.R. China and.
b Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife , College of Life Sciences, Sichuan University , Sichuan , P.R. China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(1):740-1. doi: 10.3109/19401736.2014.913167. Epub 2014 Apr 29.
In this study, the complete mitochondrial genome of Maruca testulalis was determined (GenBank accession number KJ623250). It is 15,110 bp in length, and included 13 PCGs, 2 rRNA gens, 22 tRNA genes, and a A + T-rich region. Its gene order and orientation are identical to other crambid species. The AT-skew and GC-skew of the entire mitogenome are negative and the nucleotide composition is biased toward A + T nucleotides (80.6%). All PCGs begin with ATN codons, except for COI which is initiated by CGA. All the 22 tRAN genes can be fold into the typical clover-leaf secondary structure except for tRNA(ser)(AGN). The 335 bp long A + T-rich region is located between srRNA and tRNA(Met) and contains some common features of the other lepidopterans, including the motif ATAGT followed by 14 bp poly-T stretches and a microsatellite-like (AT)11 elements preceded by the ATTTA motif.
在本研究中,测定了豆野螟(Maruca testulalis)的完整线粒体基因组(GenBank登录号KJ623250)。其长度为15,110 bp,包括13个蛋白质编码基因(PCGs)、2个核糖体RNA基因、22个转运RNA基因和一个富含A + T的区域。其基因顺序和方向与其他草螟科物种相同。整个线粒体基因组的AT偏斜和GC偏斜均为负值,核苷酸组成偏向于A + T核苷酸(80.6%)。除了由CGA起始的细胞色素氧化酶亚基I(COI)外,所有PCGs均以ATN密码子起始。除了丝氨酸转运RNA(AGN)外,所有22个转运RNA基因均可折叠成典型的三叶草二级结构。富含A + T的335 bp区域位于小核糖体RNA和甲硫氨酸转运RNA(tRNA(Met))之间,包含其他鳞翅目昆虫的一些共同特征,包括基序ATAGT,其后跟着14 bp的多聚T序列,以及在ATTTA基序之前的类似微卫星的(AT)11元件。