Lin X H, Li W Y
Shi Yan Sheng Wu Xue Bao. 1989 Jun;22(2):205-11.
Using rat AFP cDNA clone pRAF87 and subcloned 5' end fragment of AFP gene as probes, we have analysed the DNaseI sensitivity and DNaseI hypersensitive sites of AFP gene in chromatin of rat fetal liver (active transcription), adult liver (potentially active transcription) and adult kidney (inactive transcription). The results indicated that fetal liver AFP genes are much more sensitive than their counter parts. There are three DNaseI hypersensitive sites in the fetal AFP gene, namely, upstream -3kb region, 1.37kb region just around transcription start sites and the last located in downstream 4.1 kb EcoR I fragment. The 1.37 kb DNasI hypersensitive site also exists in adult liver. The relationship between AFP gene DNaseI sensitivity, DNaseI hypersensitive sites and AFP gene expression have been discussed.
利用大鼠甲胎蛋白（AFP）cDNA克隆pRAF87及AFP基因5′端亚克隆片段作探针，我们分析了大鼠胎肝（活跃转录）、成体肝（潜在活跃转录）及成体肾（非活跃转录）染色质中AFP基因的DNaseI敏感性及DNaseI超敏感位点。结果表明，胎肝AFP基因比其相应的其他组织基因更敏感。胎肝AFP基因有三个DNaseI超敏感位点，即上游-3kb区域、转录起始位点附近的1.37kb区域以及位于下游4.1kb EcoR I片段中的最后一个位点。成体肝中也存在1.37kb的DNaseI超敏感位点。本文还讨论了AFP基因DNaseI敏感性、DNaseI超敏感位点与AFP基因表达之间的关系。
