Egelrud T, Stigbrand T
Department of Dermatology, University of Umeå, Sweden.
Acta Derm Venereol. 1989;69(5):373-9.
The cytokeratin composition of palmo-plantar epidermis from sites with different degrees of mechanically induced thickening of the stratum corneum was analysed. The urea-soluble proteins of the stratum corneum were analysed by two-dimensional electrophoresis. Viable epidermal layers were analysed by immunofluorescence microscopy with polyclonal and monoclonal antibodies. A mouse monoclonal antibody specific for cytokeratin no. 9 was prepared for the study. Significant amounts of low molecular weight cytokeratins were found in suprabasal layers at sites with the most pronounced thickening of the stratum corneum. This was taken as evidence that palmo-plantar epidermis responds to mechanical stress with hyperproliferation. At sites where stratum corneum thickness is most increased this hyperproliferation appears to involve two different populations of cells--one capable of expressing high molecular weight, differentiation-related cytokeratins in the suprabasal epidermal layers, and one population that does not express these cytokeratins. At sites with intermediate epidermal hyperplasticity the high molecular weight cytokeratins were predominant in all suprabasal cells.
对来自角质层机械性增厚程度不同部位的掌跖表皮的细胞角蛋白组成进行了分析。通过二维电泳分析角质层的尿素可溶性蛋白。用多克隆和单克隆抗体通过免疫荧光显微镜对活表皮层进行分析。为该研究制备了一种对细胞角蛋白9特异的小鼠单克隆抗体。在角质层增厚最明显部位的基底层上部发现了大量低分子量细胞角蛋白。这被视为掌跖表皮以细胞过度增殖对机械应力作出反应的证据。在角质层厚度增加最明显的部位，这种细胞过度增殖似乎涉及两种不同的细胞群体——一种能够在基底层上部的表皮层表达高分子量、与分化相关的细胞角蛋白，另一种群体则不表达这些细胞角蛋白。在表皮中度增生的部位，高分子量细胞角蛋白在所有基底层上部细胞中占主导地位。
