a Department of Veterinary Public Health, Laboratory for Residue Control , Croatian Veterinary Institute , Zagreb , Croatia.
Food Addit Contam Part B Surveill. 2013;6(2):90-7. doi: 10.1080/19393210.2012.727474. Epub 2012 Oct 5.
In total 307 egg and 275 liver samples were examined for nicarbazin and 365 eggs for diclazuril over a 30-month period. Enzyme-linked immunosorbent assay methods used for quantification were validated according to European Commission Decision 2002/657/EC. Non-compliant samples were confirmed by LC-MS/MS. Mean diclazuril concentrations in egg samples were 0.31 µg kg⁻¹, which is below the MRL. In only one egg sample, 2.26 µg kg⁻¹ was determined by enzyme-linked immunosorbent assay, although confirmation by LC-MS/MS gave a value of 1.6 µg kg(-1). Mean nicarbazin levels determined were 1.85 µg kg⁻¹ in egg and 21.1 µg kg⁻¹ in liver samples. Four samples, one egg and three livers, yielded elevated concentrations of nicarbazin, but only in the egg sample the LC-MS/MS method confirmed nicarbazin (106 µg kg⁻¹) above the MRL value.
在 30 个月的时间里，共检测了 307 个鸡蛋和 275 个肝脏样本中的尼卡巴嗪，以及 365 个鸡蛋中的地克珠利。用于定量的酶联免疫吸附测定方法根据欧洲委员会第 2002/657/EC 号决定进行了验证。不符合要求的样本通过 LC-MS/MS 进行了确认。鸡蛋样本中地克珠利的平均浓度为 0.31μg/kg，低于最大残留限量。仅在一个鸡蛋样本中，通过酶联免疫吸附测定法测定为 2.26μg/kg，但通过 LC-MS/MS 确认的值为 1.6μg/kg。鸡蛋和肝脏样本中分别测定的尼卡巴嗪平均水平为 1.85μg/kg 和 21.1μg/kg。有四个样本，一个鸡蛋和三个肝脏，其尼卡巴嗪浓度升高，但只有在鸡蛋样本中，LC-MS/MS 方法确认尼卡巴嗪（106μg/kg）超过了最大残留限量值。
