Department of Plant Pathology and the Genome Center, University of California Davis, Davis, CA 95616, USA; Department of Bioresources Engineering, Sejong University, Seoul 143-747, Republic of Korea.
Graduate School of Biotechnology & Crop Biotech Institute, Kyung Hee University, Yongin 446-701, Republic of Korea.
Biochem Biophys Res Commun. 2014 May 23;448(1):70-5. doi: 10.1016/j.bbrc.2014.04.093. Epub 2014 Apr 26.
Plant innate immunity is mediated by pattern recognition receptors (PRRs) and intracellular NB-LRR (nucleotide-binding domain and leucine-rich repeat) proteins. Overexpression of the endoplasmic reticulum (ER) chaperone, luminal-binding protein 3 (BiP3) compromises resistance to Xanthomonas oryzae pv. oryzae (Xoo) mediated by the rice PRR XA21 [12]. Here we show that BiP3 overexpression also compromises resistance mediated by rice XA3, a PRR that provides broad-spectrum resistance to Xoo. In contrast, BiP3 overexpression has no effect on resistance mediated by rice Pi5, an NB-LRR protein that confers resistance to the fungal pathogen Magnaporthe oryzae (M. oryzae). Our results suggest that rice BiP3 regulates membrane-resident PRR-mediated immunity.
植物先天免疫由模式识别受体（PRRs）和细胞内的核苷酸结合域和富含亮氨酸重复（NB-LRR）蛋白介导。内质网（ER）伴侣蛋白内质网腔结合蛋白 3（BiP3）的过表达会削弱水稻 PRR XA21 介导的对稻黄单胞菌 pv.oryzae（Xoo）的抗性[12]。在这里，我们表明 BiP3 的过表达也会削弱由水稻 XA3 介导的抗性，XA3 是一种提供广谱抗 Xoo 的 PRR。相比之下，BiP3 的过表达对水稻 Pi5 介导的抗性没有影响，Pi5 是一种 NB-LRR 蛋白，可赋予对真菌病原体稻瘟病菌（M.oryzae）的抗性。我们的结果表明，水稻 BiP3 调节膜驻留 PRR 介导的免疫。
