Salim Chinnu, Rajini P S
Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, New Delhi, India; Food Protectants and Infestation Control Department, CSIR-Central Food Technological Research Institute, Mysore 570020, India.
Academy of Scientific and Innovative Research (AcSIR), Anusandhan Bhawan, New Delhi, India; Food Protectants and Infestation Control Department, CSIR-Central Food Technological Research Institute, Mysore 570020, India.
Physiol Behav. 2014 May 28;131:142-8. doi: 10.1016/j.physbeh.2014.04.022. Epub 2014 Apr 26.
Several studies have demonstrated that high glucose feeding induced oxidative stress and apoptosis thereby affecting growth, fertility, aging and lifespan in Caenorhabditis elegans. Earlier studies from our laboratory had clearly established the propensity of monocrotophos, an OPI to alter the physiological and behavioral responses of C. elegans. The present study was aimed to investigate the effect of monocrotophos (MCP) on physiological/behavioral and biochemical responses in C. elegans that were maintained on high glucose diet. We exposed the worms through development to high glucose diet (2%) and then treated with sublethal concentrations of MCP (0.5, 0.75, 1.5mM). We measured the behavioral responses in terms of locomotion, physiological responses in terms of egg laying, brood size, lifespan; morphological alterations; and biochemical responses including glucose content. The worms exposed from egg stage through development to high glucose diet showed enhanced toxic outcome of MCP in terms of physiological, behavioral and biochemical responses. Our studies showed that C. elegans is a good model to study glucose-OPI interactive neurotoxicity since all the responses could be studied at ease in this organism and the outcome could be well extrapolated to those that one would expect in higher animals.
多项研究表明，高糖喂养会诱导氧化应激和细胞凋亡，从而影响秀丽隐杆线虫的生长、繁殖力、衰老和寿命。我们实验室早期的研究已经明确证实了久效磷（一种有机磷杀虫剂）会改变秀丽隐杆线虫的生理和行为反应。本研究旨在调查久效磷（MCP）对以高糖饮食饲养的秀丽隐杆线虫的生理/行为和生化反应的影响。我们使线虫在发育过程中暴露于高糖饮食（2%），然后用亚致死浓度的MCP（0.5、0.75、1.5mM）进行处理。我们从运动方面测量行为反应，从产卵、产卵量、寿命方面测量生理反应；观察形态学改变；并测量包括葡萄糖含量在内的生化反应。从卵期开始在发育过程中暴露于高糖饮食的线虫，在生理、行为和生化反应方面显示出MCP更强的毒性作用。我们的研究表明，秀丽隐杆线虫是研究葡萄糖-有机磷杀虫剂相互作用神经毒性的良好模型，因为在这种生物中可以轻松研究所有反应，并且其结果可以很好地外推到高等动物身上。
