Food Protectants and Infestation Control Department, Council of Scientific and Industrial Research, Central Food Technological Research Institute, Mysore, India.
J Diabetes. 2015 Jan;7(1):47-59. doi: 10.1111/1753-0407.12158. Epub 2014 May 8.
Our earlier studies had shown that monocrotophos (MCP), an organophosphorus insecticide (OPI), has the propensity to augment the secondary complications associated with type-1 diabetes. The present study investigates whether rats exposed for prolonged periods to monocrotophos would develop insulin resistance mediated by alteration in glucose homeostasis.
Male rats were administered sublethal doses of monocrotophos daily for 180 days. Interim blood samples were collected to measure alteration in blood glucose and lipid profile. Rats were also subjected to glucose and insulin tolerance test and fasting blood glucose and insulin levels were measured to calculate insulin resistance by HOMA-IR method. After 180 days, the rats were also evaluated for pancreatic histology and activities of hepatic gluconeogenetic enzymes.
Monocrotophos elicited a gradual and sustained increase in blood glucose and insulin resistance in rats with concomitant glucose intolerance and reduced insulin sensitivity. MCP exposure was also associated with increase in weights of key white adipose pads, activities of gluconeogenesis enzymes and increase in pancreatic islet diameter, all of which led to hyperglycemia, hyperinsulinemia and dyslipidaemia.
Long-term exposure of rats to MCP resulted in glucose intolerance with hyperinsulinemia, a hallmark of insulin resistance. Our data suggest that chronic exposure to low doses of monocrotophos, might lead to development of insulin resistance by altering lipid profile and glucose homeostasis.
我们之前的研究表明，有机磷杀虫剂久效磷（MCP）有加剧 1 型糖尿病相关继发并发症的倾向。本研究旨在探究长期接触久效磷是否会通过改变葡萄糖稳态导致胰岛素抵抗。
雄性大鼠每日给予亚致死剂量的久效磷，持续 180 天。采集中间血样以测量血糖和血脂谱的变化。还对大鼠进行葡萄糖和胰岛素耐量试验，测量空腹血糖和胰岛素水平，通过 HOMA-IR 法计算胰岛素抵抗指数。180 天后，还评估大鼠的胰腺组织学和肝糖异生酶活性。
久效磷可引起大鼠血糖逐渐持续升高和胰岛素抵抗，伴有葡萄糖耐量降低和胰岛素敏感性降低。MCP 暴露还与关键白色脂肪垫重量增加、糖异生酶活性增加以及胰岛直径增加有关，所有这些都导致高血糖、高胰岛素血症和血脂异常。
大鼠长期接触 MCP 会导致葡萄糖耐量降低伴高胰岛素血症，这是胰岛素抵抗的标志。我们的数据表明，慢性接触低剂量的久效磷可能会通过改变脂质谱和葡萄糖稳态导致胰岛素抵抗的发展。
