Salunke Balwant P, Umathe Sudhir N, Chavan Jagatpalsingh G
Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Mahatma Jyotiba Fuley Shaikshanik Parisar, Amravati Road, Nagpur, MS,440 033, India.
Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Mahatma Jyotiba Fuley Shaikshanik Parisar, Amravati Road, Nagpur, MS,440 033, India.
Pharmacol Biochem Behav. 2014 Jul;122:273-8. doi: 10.1016/j.pbb.2014.04.007. Epub 2014 Apr 26.
It is well documented that extremely low frequency magnetic field (ELF MF) produced effects on the function of nervous system in humans and laboratory animals. Dopaminergic and serotonergic pathways have been implicated in obsessive compulsive disorder (OCD). Recently involvement of nitric oxide (NO) in OCD-like behavior is suggested. Hence, the present study was carried out to understand the involvement of dopamine, serotonin and NO in ELF MF induced OCD-like behavior. Swiss albino mice were exposed to ELF MF (50 Hz, 10G) for 8 h/day for 7, 30, 60, 90 and 120 days by subjecting them to Helmholtz coils. OCD-like behavior was assessed in terms of marble burying behavior (MBB) test. Results revealed that ELF MF induced time dependant MBB, on 7th, 30th, 60th, 90th, and 120th exposure day. Further, levels of dopamine, serotonin and NO after 120 days of ELF MF exposure were determined in the regions of the brain. The neurohumoral studies revealed that exposure to ELF MF increased NO levels in cortex, hippocampus and hypothalamus, and levels of dopamine and serotonin remain unaffected. As OCD-like behavior after ELF MF exposure was associated with higher levels of NO with no significant change in serotonin and dopamine. The effect of such exposure was studied in groups concurrently treated with NO modulators, NO precursor, L-ARG (400 mg/kg) or NOS inhibitor, L-NAME (15.0mg/kg) or 7-NI (10.0 mg/kg). These treatments revealed that NO precursor exacerbated and NOS inhibitors attenuated ELF MF induced OCD-like behavior with corresponding changes in the levels of NO.
有充分文献记载，极低频磁场（ELF MF）会对人类和实验动物的神经系统功能产生影响。多巴胺能和5-羟色胺能通路与强迫症（OCD）有关。最近有研究表明一氧化氮（NO）参与了类似OCD的行为。因此，本研究旨在了解多巴胺、5-羟色胺和NO在ELF MF诱导的类似OCD行为中的作用。通过让瑞士白化小鼠暴露于亥姆霍兹线圈产生的ELF MF（50Hz，10G）中，每天暴露8小时，持续7、30、60、90和120天。通过大理石埋藏行为（MBB）试验评估类似OCD的行为。结果显示，在第7、30、60、90和120天的暴露日，ELF MF诱导了时间依赖性的MBB。此外，在ELF MF暴露120天后，测定了大脑各区域多巴胺、5-羟色胺和NO的水平。神经体液研究表明，暴露于ELF MF会增加皮质、海马体和下丘脑的NO水平，而多巴胺和5-羟色胺的水平未受影响。由于ELF MF暴露后的类似OCD行为与较高的NO水平相关，而5-羟色胺和多巴胺没有显著变化。在同时用NO调节剂、NO前体L-ARG（400mg/kg）或NOS抑制剂L-NAME（15.0mg/kg）或7-NI（10.0mg/kg）处理的组中研究了这种暴露的影响。这些处理表明，NO前体加剧了ELF MF诱导的类似OCD行为，而NOS抑制剂减弱了这种行为，同时NO水平也有相应变化。
