Madjid Ansari Alireza, Farzampour Shahrokh, Sadr Ali, Shekarchi Babak, Majidzadeh-A Keivan
Cancer Alternative and Complementary Medicine Department, Breast Cancer Research Center, ACECR, Iran.
Tasnim Biotechnology Research Center, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran.
Life Sci. 2016 Feb 1;146:52-7. doi: 10.1016/j.lfs.2015.12.055. Epub 2016 Jan 5.
Previous reports on the possible effects of Extremely Low Frequency Magnetic Fields (ELF MF) on mood have been paradoxical in different settings while no study has yet been conducted on animal behavior. In addition, it was shown that ELF MF exposure makes an increase in brain nitric oxide level. Therefore, in the current study, we aimed to assess the possible effect(s) of ELF MF exposure on mice Forced Swimming Test (FST) and evaluate the probable role of the increased level of nitric oxide in the observed behavior.
Male adult mice NMRI were recruited to investigate the short term and long term ELF MF exposure (0.5 mT and 50 Hz, single 2h and 2 weeks 2h a day). Locomotor behavior was assessed by using open-field test (OFT) followed by FST to evaluate the immobility time. Accordingly, NΩ-nitro-l-arginine methyl ester 30 mg/kg was used to exert anti-depressant like effect.
According to the results, short term exposure did not alter the immobility time, whereas long term exposure significantly reduces immobility time (p<0.01). However, it was revealed that the locomotion did not differ among all experimental groups. Short term exposure reversed the anti-depressant like effect resulting from 30 mg/kg of NΩ-nitro-l-arginine methyl ester (p<0.01).
It has been concluded that long term exposure could alter the depressive disorder in mice, whereas short term exposure has no significant effect. Also, reversing the anti-depressant activity of L-NAME indicates a probable increase in the brain nitric oxide.
先前关于极低频磁场（ELF MF）对情绪可能产生的影响的报告在不同环境中相互矛盾，且尚未有关于动物行为的研究。此外，有研究表明暴露于ELF MF会使大脑一氧化氮水平升高。因此，在本研究中，我们旨在评估暴露于ELF MF对小鼠强迫游泳试验（FST）的可能影响，并评估一氧化氮水平升高在观察到的行为中可能发挥的作用。
招募雄性成年NMRI小鼠，研究短期和长期ELF MF暴露（0.5 mT和50 Hz，单次2小时以及每天2小时，持续2周）。通过旷场试验（OFT）评估运动行为，随后进行FST以评估不动时间。相应地，使用30 mg/kg的NΩ-硝基-L-精氨酸甲酯来发挥类似抗抑郁的作用。
根据结果，短期暴露并未改变不动时间，而长期暴露显著缩短了不动时间（p<0.01）。然而，结果显示所有实验组之间的运动能力并无差异。短期暴露逆转了30 mg/kg的NΩ-硝基-L-精氨酸甲酯所产生的类似抗抑郁作用（p<0.01）。
得出的结论是，长期暴露可改变小鼠的抑郁障碍，而短期暴露无显著影响。此外，L-NAME抗抑郁活性的逆转表明大脑一氧化氮可能增加。
