Manzini S, Conti S, Maggi C A, Abelli L, Somma V, Del Bianco E, Geppetti P
Pharmacology Department, Malesci S.p.A., Florence, Italy.
Am Rev Respir Dis. 1989 Oct;140(4):936-41. doi: 10.1164/ajrccm/140.4.936.
The motor and inflammatory effects of capsaicin, substance P, and neurokinin A, as well as the content and release of substance P-like immunoreactivity (SP-LI), were assessed in the upper and lower guinea pig trachea and in the main bronchus. Capsaicin-induced motor and inflammatory effects were greater in the lower than in the upper tract of the trachea and much more evident at the bronchial than at the tracheal level. On the other hand, no significant regional differences were observed in the potency and efficacy of tachykinins. SP-LI content was significantly greater in the lower than in the upper tract of the trachea and about five times greater in the bronchus than in the trachea. In each single tracheal preparation, a highly significant correlation was found between the motor effect of capsaicin (but not carbachol) and the SP-LI content. Furthermore, capsaicin-induced release of SP-LI was about eight times greater in bronchial than in tracheal tissues. In view of the strict correlation observed between magnitude of motor and inflammatory actions of capsaicin (but not substance P or neurokinin A) and SP-LI content and release, it is proposed that the regional differences in the response of guinea pig respiratory tissues to activation of capsaicin-sensitive sensory fibers might be mainly prejunctional in origin.
在豚鼠的上、下气管及主支气管中，评估了辣椒素、P物质和神经激肽A的运动和炎症效应，以及P物质样免疫反应性（SP-LI）的含量和释放。辣椒素诱导的运动和炎症效应在气管下段比上段更明显，在支气管水平比气管水平更显著。另一方面，速激肽的效力和功效未观察到明显的区域差异。气管下段的SP-LI含量显著高于上段，支气管中的含量约为气管中的五倍。在每个单一的气管标本中，发现辣椒素（而非卡巴胆碱）的运动效应与SP-LI含量之间存在高度显著的相关性。此外，辣椒素诱导的支气管组织中SP-LI的释放量约为气管组织中的八倍。鉴于观察到辣椒素（而非P物质或神经激肽A）的运动和炎症作用强度与SP-LI含量及释放之间存在严格的相关性，推测豚鼠呼吸组织对辣椒素敏感感觉纤维激活反应的区域差异可能主要源于节前。
