Chomik M
Department of Immunology of Infectious Diseases, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław.
Arch Immunol Ther Exp (Warsz). 1988;36(5):547-53.
Experiments were carried out with A/USSR/053/74/H3N2 influenza virus. To remove lipids from virus particles, the enzyme phospholipase C, butanol-ether and the detergent cytylpyridinum chloride were used. Mild treatment of the influenza virus with phospholipase C resulted in a slight decrease in the activity of both surface antigens of the virions as well as in a suppression of their infectivity. Such virus is capable of IFN induction in mouse. Long-term (25 h) treatment of the virus with phospholipase C caused gross destruction of virions. Thus, despite the preserved neuraminidase activity the preparation was no longer capable of IFN induction. The treatment with butanol-ether and the detergent cytylpyridinum chloride brought similar results--fully preserved hemagglutination and neuraminidase activity and a complete loss of IFN induction.
用A/苏联/053/74/H3N2流感病毒进行了实验。为了从病毒颗粒中去除脂质，使用了磷脂酶C、丁醇-乙醚和去污剂氯化十六烷基吡啶。用磷脂酶C对流感病毒进行温和处理，导致病毒颗粒两种表面抗原的活性略有下降，并抑制其感染性。这种病毒能够在小鼠中诱导干扰素。用磷脂酶C对病毒进行长期（25小时）处理会导致病毒颗粒严重破坏。因此，尽管保留了神经氨酸酶活性，但该制剂不再能够诱导干扰素。用丁醇-乙醚和去污剂氯化十六烷基吡啶处理也得到了类似的结果——血凝和神经氨酸酶活性完全保留，但诱导干扰素的能力完全丧失。
