评估erythro-9(2-羟基-3-壬基)腺嘌呤（EHNA）对体外HIV-1产生的影响。

Evaluation of the effects of erythro-9(2-hydroxy-3-nonyl) adenine (EHNA) on HIV-1 production in vitro.

作者信息

Sei Y, Inoue M, Tsuboi I, Yokoyama M M, Arora P K

机构信息

Laboratory of Neuroscience, NIDDK, Bethesda, MD 20892.

出版信息

Biochem Biophys Res Commun. 1989 Oct 16;164(1):345-50. doi: 10.1016/0006-291x(89)91724-5.

DOI:10.1016/0006-291x(89)91724-5

PMID:2478129

Abstract

Effects of erythro-9(2-hydroxy-3-nonyl) adenine (EHNA), an inhibitor of the common Adenosine deaminase (adenosine aminohydrolase, EC 3.5.4.4.), on HIV-1 production was evaluated in vitro. Reverse transcriptase (RT) activity in the supernatant was inhibited by nearly 50% in EHNA-treated HIV-1 infected H9 cells, when compared with untreated but infected H9 cells. There was also a significant decrease in cell viability, but this was reversed following the addition of deoxycytidine (dC) to these cultures. The combined treatment was also effective in suppressing HIV-1 release from HIV-1-infected U937 cells. This combined EHNA plus dC treatment had no effect on RT activity in the cell lysates, suggesting that the inhibition of HIV-1 production may be due to the disturbance of virus release from infected cells.

摘要

体外评估了常见腺苷脱氨酶（腺苷氨基水解酶，EC 3.5.4.4.）抑制剂erythro-9(2-羟基-3-壬基)腺嘌呤（EHNA）对HIV-1产生的影响。与未处理但感染的H9细胞相比，在经EHNA处理的HIV-1感染的H9细胞中，上清液中的逆转录酶（RT）活性被抑制了近50%。细胞活力也有显著下降，但在向这些培养物中添加脱氧胞苷（dC）后，这种情况得到了逆转。联合治疗在抑制HIV-1从HIV-1感染的U937细胞中释放方面也有效。这种EHNA加dC的联合治疗对细胞裂解物中的RT活性没有影响，这表明对HIV-1产生的抑制可能是由于受感染细胞释放病毒受到干扰。